Labeling embryonic mouse central nervous system cells by in utero electroporation

Hidenori Tabata, Kazunori Nakajima

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

During cerebral development, neurons are generated near the ventricle and then migrate toward the pial surface. In this review, we describe the method of in utero electroporation, this method allows the morphology of the migrating neurons to be visualized and the effect of overexpression or knock down of any gene to be examined. After electroporation of a green fluorescent protein (GFP) expression vector by this method, GFP-positive cells are first found in the ventricular zone, and their distribution then gradually shift toward the pial surface. A few days later, most of the GFP positive cells were aligned beneath the marginal zone, with the normal course of cortical neuronal migration.

Original languageEnglish
Pages (from-to)507-511
Number of pages5
JournalDevelopment Growth and Differentiation
Volume50
Issue number6
DOIs
Publication statusPublished - 2008 Aug

Fingerprint

Electroporation
Green Fluorescent Proteins
Central Nervous System
Gene Knockdown Techniques
Neurons

Keywords

  • Cerebral cortex
  • Gene transfer
  • Neuronal migration

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Labeling embryonic mouse central nervous system cells by in utero electroporation. / Tabata, Hidenori; Nakajima, Kazunori.

In: Development Growth and Differentiation, Vol. 50, No. 6, 08.2008, p. 507-511.

Research output: Contribution to journalArticle

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