Abstract
Background & Aims: Previous studies have linked Crohn's disease (CD) to the pericentromeric region of chromosome 16 (IBD1). Three independent studies of Western populations have recently shown that 3 variants of NOD2, a gene located at 16q12, are associated with susceptibility to CD. Here, we have evaluated the 3 NOD2 variants in Japanese patients to determine whether the gene is also associated with susceptibility to CD in a non-Western population. Methods: Blood samples were obtained from 350 patients with CD, 272 patients with ulcerative colitis, and 292 healthy controls at 3 hospitals in Japan. DNA was sequenced in the region of the 3 NOD2 variants (C2104T in exon 4, G2722C in exon 8, and 3020insC in exon 11) by genomic polymerase chain reaction followed by direct sequencing. Results: Among the subjects in our 3 study groups, including patients with CD, patients with ulcerative colitis, and healthy controls, none had common NOD2 variants that have been associated with CD in white patients. Conclusions: These results indicate that genetic variation, which may predispose some human populations to CD, may not be present in other populations and specifically that common variants in NOD2 found in white patients With CD are not associated with CD in the Japanese population.
| Original language | English |
|---|---|
| Pages (from-to) | 86-91 |
| Number of pages | 6 |
| Journal | Gastroenterology |
| Volume | 123 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2002 |
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ASJC Scopus subject areas
- Gastroenterology
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Lack of common NOD2 variants in Japanese patients with Crohn's disease. / Inoue, Nagamu; Tamura, Kazuo; Kinouchi, Yoshitaka; Fukuda, Yoshihiro; Takahashi, Seiichi; Ogura, Yasunori; Inohara, Naohiro; Núez, Gabriel; Kishi, Yusuke; Koike, Yuji; Shimosegawa, Tooru; Shimoyama, Takashi; Hibi, Toshifumi.
In: Gastroenterology, Vol. 123, No. 1, 2002, p. 86-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of common NOD2 variants in Japanese patients with Crohn's disease
AU - Inoue, Nagamu
AU - Tamura, Kazuo
AU - Kinouchi, Yoshitaka
AU - Fukuda, Yoshihiro
AU - Takahashi, Seiichi
AU - Ogura, Yasunori
AU - Inohara, Naohiro
AU - Núez, Gabriel
AU - Kishi, Yusuke
AU - Koike, Yuji
AU - Shimosegawa, Tooru
AU - Shimoyama, Takashi
AU - Hibi, Toshifumi
PY - 2002
Y1 - 2002
N2 - Background & Aims: Previous studies have linked Crohn's disease (CD) to the pericentromeric region of chromosome 16 (IBD1). Three independent studies of Western populations have recently shown that 3 variants of NOD2, a gene located at 16q12, are associated with susceptibility to CD. Here, we have evaluated the 3 NOD2 variants in Japanese patients to determine whether the gene is also associated with susceptibility to CD in a non-Western population. Methods: Blood samples were obtained from 350 patients with CD, 272 patients with ulcerative colitis, and 292 healthy controls at 3 hospitals in Japan. DNA was sequenced in the region of the 3 NOD2 variants (C2104T in exon 4, G2722C in exon 8, and 3020insC in exon 11) by genomic polymerase chain reaction followed by direct sequencing. Results: Among the subjects in our 3 study groups, including patients with CD, patients with ulcerative colitis, and healthy controls, none had common NOD2 variants that have been associated with CD in white patients. Conclusions: These results indicate that genetic variation, which may predispose some human populations to CD, may not be present in other populations and specifically that common variants in NOD2 found in white patients With CD are not associated with CD in the Japanese population.
AB - Background & Aims: Previous studies have linked Crohn's disease (CD) to the pericentromeric region of chromosome 16 (IBD1). Three independent studies of Western populations have recently shown that 3 variants of NOD2, a gene located at 16q12, are associated with susceptibility to CD. Here, we have evaluated the 3 NOD2 variants in Japanese patients to determine whether the gene is also associated with susceptibility to CD in a non-Western population. Methods: Blood samples were obtained from 350 patients with CD, 272 patients with ulcerative colitis, and 292 healthy controls at 3 hospitals in Japan. DNA was sequenced in the region of the 3 NOD2 variants (C2104T in exon 4, G2722C in exon 8, and 3020insC in exon 11) by genomic polymerase chain reaction followed by direct sequencing. Results: Among the subjects in our 3 study groups, including patients with CD, patients with ulcerative colitis, and healthy controls, none had common NOD2 variants that have been associated with CD in white patients. Conclusions: These results indicate that genetic variation, which may predispose some human populations to CD, may not be present in other populations and specifically that common variants in NOD2 found in white patients With CD are not associated with CD in the Japanese population.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036306951&partnerID=8YFLogxK
U2 - 10.1053/gast.2002.34155
DO - 10.1053/gast.2002.34155
M3 - Article
C2 - 12105836
AN - SCOPUS:0036306951
VL - 123
SP - 86
EP - 91
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 1
ER -