Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain

Shinichi Takahashi, M. Cook, J. Jehle, C. Kennedy, L. Sokoloff

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The effects of chronic treatment with N(G)-nitro-L-arginine methyl ester, a potent inhibitor of nitric oxide synthase activity, on local cerebral glucose utilization were examined in conscious rats. Intraperitoneal injections of 50 mg/kg of the nitroarginine twice daily for 4 days have been found to result in almost complete inhibition of nitric oxide synthase activity in brain. Local cerebral glucose utilization was determined by means of the quantitative autoradiographic [14C] deoxyglucose method in en experimental group (n = 7) of rats that were treated with the nitroarginine according to this schedule and in a normal control group (n = 7) treated similarly with saline. The rats were conscious but partially restrained during the determinations of local cerebral glucose utilization. The nitroarginine treatment raised mean arterial blood pressure statistically significantly to 147 ± 3 mm Hg (mean ± SEM) from a level of 120 ± 5 mm Hg in the saline controls (p < 0.001 by grouped t test), but there were no statistically significant effects on glucose utilization in any of 39 brain structures examined. It is concluded that nitric oxide normally exerts no significant influence on energy metabolism in the rat brain.

Original languageEnglish
Pages (from-to)414-419
Number of pages6
JournalJournal of Neurochemistry
Volume65
Issue number1
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Rats
Nitroarginine
Brain
Nitric Oxide
Glucose
Nitric Oxide Synthase
Arterial Pressure
NG-Nitroarginine Methyl Ester
Blood pressure
Deoxyglucose
Intraperitoneal Injections
Energy Metabolism
Appointments and Schedules
Control Groups
Scanning electron microscopy
Therapeutics

Keywords

  • [C] Deoxyglucose
  • Cerebral energy metabolism
  • N(G)-Nitro-L-arginine methyl ester
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Takahashi, S., Cook, M., Jehle, J., Kennedy, C., & Sokoloff, L. (1995). Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain. Journal of Neurochemistry, 65(1), 414-419.

Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain. / Takahashi, Shinichi; Cook, M.; Jehle, J.; Kennedy, C.; Sokoloff, L.

In: Journal of Neurochemistry, Vol. 65, No. 1, 1995, p. 414-419.

Research output: Contribution to journalArticle

Takahashi, S, Cook, M, Jehle, J, Kennedy, C & Sokoloff, L 1995, 'Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain', Journal of Neurochemistry, vol. 65, no. 1, pp. 414-419.
Takahashi, Shinichi ; Cook, M. ; Jehle, J. ; Kennedy, C. ; Sokoloff, L. / Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain. In: Journal of Neurochemistry. 1995 ; Vol. 65, No. 1. pp. 414-419.
@article{7b491c8ea1b54f65ba99f6efb0c83308,
title = "Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain",
abstract = "The effects of chronic treatment with N(G)-nitro-L-arginine methyl ester, a potent inhibitor of nitric oxide synthase activity, on local cerebral glucose utilization were examined in conscious rats. Intraperitoneal injections of 50 mg/kg of the nitroarginine twice daily for 4 days have been found to result in almost complete inhibition of nitric oxide synthase activity in brain. Local cerebral glucose utilization was determined by means of the quantitative autoradiographic [14C] deoxyglucose method in en experimental group (n = 7) of rats that were treated with the nitroarginine according to this schedule and in a normal control group (n = 7) treated similarly with saline. The rats were conscious but partially restrained during the determinations of local cerebral glucose utilization. The nitroarginine treatment raised mean arterial blood pressure statistically significantly to 147 ± 3 mm Hg (mean ± SEM) from a level of 120 ± 5 mm Hg in the saline controls (p < 0.001 by grouped t test), but there were no statistically significant effects on glucose utilization in any of 39 brain structures examined. It is concluded that nitric oxide normally exerts no significant influence on energy metabolism in the rat brain.",
keywords = "[C] Deoxyglucose, Cerebral energy metabolism, N(G)-Nitro-L-arginine methyl ester, Nitric oxide synthase",
author = "Shinichi Takahashi and M. Cook and J. Jehle and C. Kennedy and L. Sokoloff",
year = "1995",
language = "English",
volume = "65",
pages = "414--419",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Lack of effects of inhibition of nitric oxide synthesis on local glucose utilization in the rat brain

AU - Takahashi, Shinichi

AU - Cook, M.

AU - Jehle, J.

AU - Kennedy, C.

AU - Sokoloff, L.

PY - 1995

Y1 - 1995

N2 - The effects of chronic treatment with N(G)-nitro-L-arginine methyl ester, a potent inhibitor of nitric oxide synthase activity, on local cerebral glucose utilization were examined in conscious rats. Intraperitoneal injections of 50 mg/kg of the nitroarginine twice daily for 4 days have been found to result in almost complete inhibition of nitric oxide synthase activity in brain. Local cerebral glucose utilization was determined by means of the quantitative autoradiographic [14C] deoxyglucose method in en experimental group (n = 7) of rats that were treated with the nitroarginine according to this schedule and in a normal control group (n = 7) treated similarly with saline. The rats were conscious but partially restrained during the determinations of local cerebral glucose utilization. The nitroarginine treatment raised mean arterial blood pressure statistically significantly to 147 ± 3 mm Hg (mean ± SEM) from a level of 120 ± 5 mm Hg in the saline controls (p < 0.001 by grouped t test), but there were no statistically significant effects on glucose utilization in any of 39 brain structures examined. It is concluded that nitric oxide normally exerts no significant influence on energy metabolism in the rat brain.

AB - The effects of chronic treatment with N(G)-nitro-L-arginine methyl ester, a potent inhibitor of nitric oxide synthase activity, on local cerebral glucose utilization were examined in conscious rats. Intraperitoneal injections of 50 mg/kg of the nitroarginine twice daily for 4 days have been found to result in almost complete inhibition of nitric oxide synthase activity in brain. Local cerebral glucose utilization was determined by means of the quantitative autoradiographic [14C] deoxyglucose method in en experimental group (n = 7) of rats that were treated with the nitroarginine according to this schedule and in a normal control group (n = 7) treated similarly with saline. The rats were conscious but partially restrained during the determinations of local cerebral glucose utilization. The nitroarginine treatment raised mean arterial blood pressure statistically significantly to 147 ± 3 mm Hg (mean ± SEM) from a level of 120 ± 5 mm Hg in the saline controls (p < 0.001 by grouped t test), but there were no statistically significant effects on glucose utilization in any of 39 brain structures examined. It is concluded that nitric oxide normally exerts no significant influence on energy metabolism in the rat brain.

KW - [C] Deoxyglucose

KW - Cerebral energy metabolism

KW - N(G)-Nitro-L-arginine methyl ester

KW - Nitric oxide synthase

UR - http://www.scopus.com/inward/record.url?scp=0029011963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029011963&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 414

EP - 419

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 1

ER -