Lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni

Keita Nishiyama, Yasuyuki Seto, Kazuki Yoshioka, Tsutomu Kakuda, Shinji Takai, Yuji Yamamoto, Takao Mukai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Campylobacter is a normal inhabitant of the chicken gut. Pathogenic infection with this organism in humans is accompanied by severe inflammation of the intestinal mucosal surface. The aim of this study was to evaluate the ability of Lactobacillus gasseri SBT2055 (LG2055) to inhibit the adhesion and invasion of Campylobacter jejuni in vitro and to suppress C. jejuni colonization of chicks in vivo. Pretreatment with LG2055 significantly reduced adhesion to and invasion of a human epithelial cell line, Intestine 407, by C. jejuni 81-176. Methanol (MeOH)-fixed LG2055 also reduced infection by C. jejuni 81-176. However, proteinase K (ProK)-Treated LG2055 eliminated the inhibitory effects. Moreover, LG2055 co-Aggregated with C. jejuni 81-176. ProK treatment prevented this co-Aggregation, indicating that the co-Aggregation phenotype mediated by the proteinaceous cell-surface components of LG2055 is important for reducing C. jejuni 81-176 adhesion and invasion. In an in vivo assay, oral doses of LG2055 were administered to chicks daily for 14 days after oral inoculation with C. jejuni 81-176. At 14 days post-inoculation, chicks treated with LG2055 had significantly reduced cecum colonization by C. jejuni. Reduction in the number of C. jejuni 81-176 cells adhering to and internalized by human epithelial cells demonstrated that LG2055 is an organism that effectively and competitively excludes C. jejuni 81-176. In addition, the results of the chick colonization assay suggest that treatment with LG2055 could be useful in suppressing C. jejuni colonization of the chicks at early growth stages.

Original languageEnglish
Article numbere108827
JournalPloS one
Volume9
Issue number9
DOIs
Publication statusPublished - 2014 Sep 1
Externally publishedYes

Fingerprint

Lactobacillus gasseri
Campylobacter jejuni
Endopeptidase K
Adhesion
Assays
Agglomeration
Infection
infection
chicks
Methanol
adhesion
epithelial cells
Epithelial Cells
Campylobacter
Cecum
organisms
assays
Cellular Structures
cecum
oral administration

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Nishiyama, K., Seto, Y., Yoshioka, K., Kakuda, T., Takai, S., Yamamoto, Y., & Mukai, T. (2014). Lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni. PloS one, 9(9), [e108827]. https://doi.org/10.1371/journal.pone.0108827

Lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni. / Nishiyama, Keita; Seto, Yasuyuki; Yoshioka, Kazuki; Kakuda, Tsutomu; Takai, Shinji; Yamamoto, Yuji; Mukai, Takao.

In: PloS one, Vol. 9, No. 9, e108827, 01.09.2014.

Research output: Contribution to journalArticle

Nishiyama, K, Seto, Y, Yoshioka, K, Kakuda, T, Takai, S, Yamamoto, Y & Mukai, T 2014, 'Lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni', PloS one, vol. 9, no. 9, e108827. https://doi.org/10.1371/journal.pone.0108827
Nishiyama, Keita ; Seto, Yasuyuki ; Yoshioka, Kazuki ; Kakuda, Tsutomu ; Takai, Shinji ; Yamamoto, Yuji ; Mukai, Takao. / Lactobacillus gasseri sbt2055 reduces infection by and colonization of campylobacter jejuni. In: PloS one. 2014 ; Vol. 9, No. 9.
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