Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates

Keita Nishiyama, Shintaro Ueno, Makoto Sugiyama, Yuji Yamamoto, Takao Mukai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lactobacillus rhamnosus GG (LGG) is a well-established probiotic strain. The beneficial properties of this strain are partially dependent on its prolonged residence in the gastrointestinal tract, and are likely influenced by its adhesion to the intestinal mucosa. The pilin SpaC subunit, located within the Spa pili structure, is the most well studied LGG adhesion factor. However, the binding epitopes of SpaC remain largely unknown. The aim of this study was to evaluate the binding properties of SpaC to the carbohydrate moieties of intestinal glycoconjugates using a recombinant SpaC protein. In a competitive enzyme-linked immunosorbent assay, SpaC binding was markedly reduced by addition of purified mucin and the mucin oligosaccharide fraction. Histochemical staining revealed that the binding of SpaC was drastically reduced by periodic acid treatment. Moreover, in the surface plasmon resonance-based Biacore assay, SpaC bound strongly to the carbohydrate moieties containing β-galactoside at the non-reducing terminus of glycolipids. We here provide the first demonstration that SpaC binds to the oligosaccharide chains of mucins, and that the carbohydrate moieties containing β-galactoside at the non-reducing termini of glycoconjugates play a crucial role in this binding. Our results demonstrate the importance of carbohydrates of SpaC for mucus interactions.

Original languageEnglish
Pages (from-to)809-815
Number of pages7
JournalAnimal science journal = Nihon chikusan Gakkaiho
Volume87
Issue number6
DOIs
Publication statusPublished - 2016 Jun 1
Externally publishedYes

Fingerprint

Fimbriae Proteins
Lactobacillus rhamnosus
glycoconjugates
Glycoconjugates
mucins
Mucins
Carbohydrates
carbohydrates
Galactosides
galactosides
Oligosaccharides
oligosaccharides
adhesion
surface plasmon resonance
Periodic Acid
Surface Plasmon Resonance
binding properties
fimbriae
glycolipids
Glycolipids

Keywords

  • adhesion
  • glycoconjugate
  • Lactobacillus rhamnosus GG
  • pilus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Lactobacillus rhamnosus GG SpaC pilin subunit binds to the carbohydrate moieties of intestinal glycoconjugates. / Nishiyama, Keita; Ueno, Shintaro; Sugiyama, Makoto; Yamamoto, Yuji; Mukai, Takao.

In: Animal science journal = Nihon chikusan Gakkaiho, Vol. 87, No. 6, 01.06.2016, p. 809-815.

Research output: Contribution to journalArticle

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