Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

Yuji Yamada, Kentaro Hozumi, Akihiro Aso, Atsushi Hotta, Kazunori Toma, Fumihiko Katagiri, Yamato Kikkawa, Motoyoshi Nomizu

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.

Original languageEnglish
Pages (from-to)4118-4125
Number of pages8
JournalBiomaterials
Volume33
Issue number16
DOIs
Publication statusPublished - 2012 Jun 1

Keywords

  • Cell culture
  • ECM (extracellular matrix)
  • Laminin
  • Peptide
  • Polysaccharide

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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  • Cite this

    Yamada, Y., Hozumi, K., Aso, A., Hotta, A., Toma, K., Katagiri, F., Kikkawa, Y., & Nomizu, M. (2012). Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering. Biomaterials, 33(16), 4118-4125. https://doi.org/10.1016/j.biomaterials.2012.02.044