Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

Yuji Yamada, Kentaro Hozumi, Akihiro Aso, Atsushi Hotta, Kazunori Toma, Fumihiko Katagiri, Yamato Kikkawa, Motoyoshi Nomizu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.

Original languageEnglish
Pages (from-to)4118-4125
Number of pages8
JournalBiomaterials
Volume33
Issue number16
DOIs
Publication statusPublished - 2012 Jun

Fingerprint

Biocompatible Materials
Laminin
Tissue Engineering
Tissue engineering
Biomaterials
Sepharose
Peptides
Polysaccharides
AG 73
Cell culture
Cell Culture Techniques
Alginate
Endothelial cells
Adhesiveness
Fibroblasts
Scaffolds (biology)
Acinar Cells
Chitosan
Salivary Glands
Monolayers

Keywords

  • Cell culture
  • ECM (extracellular matrix)
  • Laminin
  • Peptide
  • Polysaccharide

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering. / Yamada, Yuji; Hozumi, Kentaro; Aso, Akihiro; Hotta, Atsushi; Toma, Kazunori; Katagiri, Fumihiko; Kikkawa, Yamato; Nomizu, Motoyoshi.

In: Biomaterials, Vol. 33, No. 16, 06.2012, p. 4118-4125.

Research output: Contribution to journalArticle

Yamada, Y, Hozumi, K, Aso, A, Hotta, A, Toma, K, Katagiri, F, Kikkawa, Y & Nomizu, M 2012, 'Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering', Biomaterials, vol. 33, no. 16, pp. 4118-4125. https://doi.org/10.1016/j.biomaterials.2012.02.044
Yamada, Yuji ; Hozumi, Kentaro ; Aso, Akihiro ; Hotta, Atsushi ; Toma, Kazunori ; Katagiri, Fumihiko ; Kikkawa, Yamato ; Nomizu, Motoyoshi. / Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering. In: Biomaterials. 2012 ; Vol. 33, No. 16. pp. 4118-4125.
@article{82cde98dc249499b8b63e3318c05b04b,
title = "Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering",
abstract = "Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.",
keywords = "Cell culture, ECM (extracellular matrix), Laminin, Peptide, Polysaccharide",
author = "Yuji Yamada and Kentaro Hozumi and Akihiro Aso and Atsushi Hotta and Kazunori Toma and Fumihiko Katagiri and Yamato Kikkawa and Motoyoshi Nomizu",
year = "2012",
month = "6",
doi = "10.1016/j.biomaterials.2012.02.044",
language = "English",
volume = "33",
pages = "4118--4125",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "16",

}

TY - JOUR

T1 - Laminin active peptide/agarose matrices as multifunctional biomaterials for tissue engineering

AU - Yamada, Yuji

AU - Hozumi, Kentaro

AU - Aso, Akihiro

AU - Hotta, Atsushi

AU - Toma, Kazunori

AU - Katagiri, Fumihiko

AU - Kikkawa, Yamato

AU - Nomizu, Motoyoshi

PY - 2012/6

Y1 - 2012/6

N2 - Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.

AB - Cell adhesive peptides derived from extracellular matrix components are potential candidates to afford bio-adhesiveness to cell culture scaffolds for tissue engineering. Previously, we covalently conjugated bioactive laminin peptides to polysaccharides, such as chitosan and alginate, and demonstrated their advantages as biomaterials. Here, we prepared functional polysaccharide matrices by mixing laminin active peptides and agarose gel. Several laminin peptide/agarose matrices showed cell attachment activity. In particular, peptide AG73 (RKRLQVQLSIRT)/agarose matrices promoted strong cell attachment and the cell behavior depended on the stiffness of agarose matrices. Fibroblasts formed spheroid structures on the soft AG73/agarose matrices while the cells formed a monolayer with elongated morphologies on the stiff matrices. On the stiff AG73/agarose matrices, neuronal cells extended neuritic processes and endothelial cells formed capillary-like networks. In addition, salivary gland cells formed acini-like structures on the soft matrices. These results suggest that the peptide/agarose matrices are useful for both two- and three-dimensional cell culture systems as a multifunctional biomaterial for tissue engineering.

KW - Cell culture

KW - ECM (extracellular matrix)

KW - Laminin

KW - Peptide

KW - Polysaccharide

UR - http://www.scopus.com/inward/record.url?scp=84858796810&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858796810&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2012.02.044

DO - 10.1016/j.biomaterials.2012.02.044

M3 - Article

C2 - 22410171

AN - SCOPUS:84858796810

VL - 33

SP - 4118

EP - 4125

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 16

ER -