Large-area fluorescence and electron microscopic correlative imaging with multibeam scanning electron microscopy

Shinsuke Shibata, Taro Iseda, Takayuki Mitsuhashi, Atsushi Oka, Tomoko Shindo, Nobuko Moritoki, Toshihiro Nagai, Shinya Otsubo, Takashi Inoue, Erika Sasaki, Chihiro Akazawa, Takao Takahashi, Richard Schalek, Jeff W. Lichtman, Hideyuki Okano

Research output: Contribution to journalArticle

Abstract

Recent improvements in correlative light and electron microscopy (CLEM) technology have led to dramatic improvements in the ability to observe tissues and cells. Fluorescence labeling has been used to visualize the localization of molecules of interest through immunostaining or genetic modification strategies for the identification of the molecular signatures of biological specimens. Newer technologies such as tissue clearing have expanded the field of observation available for fluorescence labeling; however, the area of correlative observation available for electron microscopy (EM) remains restricted. In this study, we developed a large-area CLEM imaging procedure to show specific molecular localization in large-scale EM sections of mouse and marmoset brain. Target molecules were labeled with antibodies and sequentially visualized in cryostat sections using fluorescence and gold particles. Fluorescence images were obtained by light microscopy immediately after antibody staining. Immunostained sections were postfixed for EM, and silver-enhanced sections were dehydrated in a graded ethanol series and embedded in resin. Ultrathin sections for EM were prepared from fully polymerized resin blocks, collected on silicon wafers, and observed by multibeam scanning electron microscopy (SEM). Multibeam SEM has made rapid, large-area observation at high resolution possible, paving the way for the analysis of detailed structures using the CLEM approach. Here, we describe detailed methods for large-area CLEM in various tissues of both rodents and primates.

Original languageEnglish
Article number29
JournalFrontiers in Neural Circuits
Volume13
DOIs
Publication statusPublished - 2019 Apr 24

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Keywords

  • CLEM
  • Connectomics
  • Correlative imaging
  • Immuno-EM
  • Multibeam SEM

ASJC Scopus subject areas

  • Neuroscience (miscellaneous)
  • Sensory Systems
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

Shibata, S., Iseda, T., Mitsuhashi, T., Oka, A., Shindo, T., Moritoki, N., Nagai, T., Otsubo, S., Inoue, T., Sasaki, E., Akazawa, C., Takahashi, T., Schalek, R., Lichtman, J. W., & Okano, H. (2019). Large-area fluorescence and electron microscopic correlative imaging with multibeam scanning electron microscopy. Frontiers in Neural Circuits, 13, [29]. https://doi.org/10.3389/fncir.2019.00029