Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism

Kiriko Murakami, Eriko Tao, Yuki Ito, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima, Takahiro Sumiya, Atsushi Nakamura, Masafumi Nishizawa

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Saccharomyces cerevisiae, for centuries the yeast that has been the workhorse for the fermentative production of ethanol, is now also a model system for biological research. The recent development of chromosome-splitting techniques has enabled the manipulation of the yeast genome on a large scale, and this has allowed us to explore questions with both biological and industrial relevance, the number of genes required for growth and the genome organization responsible for the ethanol production. To approach these questions, we successively deleted portions of the yeast genome and constructed a mutant that had lost about 5% of the genome and that gave an increased yield of ethanol and glycerol while showing levels of resistance to various stresses nearly equivalent to those of the parental strain. Further systematic deletion could lead to the formation of a eukaryotic cell with a minimum set of genes exhibiting appropriately altered regulation for enhanced metabolite production.

Original languageEnglish
Pages (from-to)589-597
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume75
Issue number3
DOIs
Publication statusPublished - 2007 Jun 1

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Murakami, K., Tao, E., Ito, Y., Sugiyama, M., Kaneko, Y., Harashima, S., Sumiya, T., Nakamura, A., & Nishizawa, M. (2007). Large scale deletions in the Saccharomyces cerevisiae genome create strains with altered regulation of carbon metabolism. Applied Microbiology and Biotechnology, 75(3), 589-597. https://doi.org/10.1007/s00253-007-0859-2