Laryngeal muscle surface receptors identified using random phage library

Paul W. Flint, Zhao Bo Li, Mohamed Lehar, Koichiro Saito, Sara I. Pai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

OBJECTIVES: The ultimate goal of this study is to improve the efficiency of gene transfer in mammalian muscle by developing targeted adenoviral vectors. Altering the tropism of viral vectors to recognize tissue specific antigens is one method to achieve this goal. This approach requires identification of cell-surface receptors and the insertion of target peptide sequences into the adenoviral fiber protein. In this study, phage biopanning was performed on cultured rat skeletal and laryngeal muscle to identify cell-surface receptors. STUDY DESIGN: In vitro cell culture and in vivo animal model. METHODS: M-13 Phage biopanning was used for muscle cell-surface receptor analysis on cultured rat skeletal and laryngeal muscle. Nonbinding and binding phage to cultured skeletal and laryngeal muscle were screened for muscle specific surface peptides. In vivo studies were then performed using muscle specific phage. RESULTS: Skeletal muscle specific binding by the YASTNPM phage was observed by in vivo immunostaining. Phage titering demonstrated a 10-fold increase in skeletal muscle binding compared with nontarget tissue. A peptide sequence (NPSQVKH) specific for laryngeal muscle yielded a 10-fold increase in laryngeal muscle phage titer compared with nontarget tissue. CONCLUSIONS: These results identify muscle cell-surface receptors that may be used as potential targets for genetic modification of adenovirus tropism. Moreover, phage specificity for skeletal and laryngeal muscle indicates specific muscle groups may be targeted.

Original languageEnglish
Pages (from-to)1930-1937
Number of pages8
JournalLaryngoscope
Volume115
Issue number11
DOIs
Publication statusPublished - 2005 Nov 1

Keywords

  • Larynx
  • Muscle
  • Peptide
  • Phage display
  • Targeting

ASJC Scopus subject areas

  • Otorhinolaryngology

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    Flint, P. W., Li, Z. B., Lehar, M., Saito, K., & Pai, S. I. (2005). Laryngeal muscle surface receptors identified using random phage library. Laryngoscope, 115(11), 1930-1937. https://doi.org/10.1097/01.mlg.0000172273.98418.8d