Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations

Tomoko Betsuyaku, Robert M. Senior

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalMicron
Volume35
Issue number4
DOIs
Publication statusPublished - 2004 Jun

Keywords

  • Clara cell
  • Microarray
  • Nissl stain
  • Real-time Polymerase chain reaction

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

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