Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations

Tomoko Betsuyaku, Robert M. Senior

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalMicron
Volume35
Issue number4
DOIs
Publication statusPublished - 2004 Jun
Externally publishedYes

Fingerprint

Microdissection
Laser Capture Microdissection
epithelium
mice
Epithelium
linings
Messenger RNA
Lasers
Epithelial Cells
cells
Linings
lasers
Complementary RNA
Microarray Analysis
genes
Microarrays
Cell Count
RNA
Amplification
Genes

Keywords

  • Clara cell
  • Microarray
  • Nissl stain
  • Real-time Polymerase chain reaction

ASJC Scopus subject areas

  • Cell Biology
  • Materials Science(all)
  • Instrumentation

Cite this

Laser capture microdissection and mRNA characterization of mouse airway epithelium : Methodological considerations. / Betsuyaku, Tomoko; Senior, Robert M.

In: Micron, Vol. 35, No. 4, 06.2004, p. 229-234.

Research output: Contribution to journalArticle

Betsuyaku, Tomoko ; Senior, Robert M. / Laser capture microdissection and mRNA characterization of mouse airway epithelium : Methodological considerations. In: Micron. 2004 ; Vol. 35, No. 4. pp. 229-234.
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