Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations

Tomoko Betsuyaku; Robert M. Senior

doi:10.1016/j.micron.2003.11.015

Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations

Tomoko Betsuyaku, Robert M. Senior

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.

Original language	English
Pages (from-to)	229-234
Number of pages	6
Journal	Micron
Volume	35
Issue number	4
DOIs	https://doi.org/10.1016/j.micron.2003.11.015
Publication status	Published - 2004 Jun
Externally published	Yes

Keywords

Clara cell
Microarray
Nissl stain
Real-time Polymerase chain reaction

ASJC Scopus subject areas

Structural Biology
Materials Science(all)
Physics and Astronomy(all)
Cell Biology

Access to Document

10.1016/j.micron.2003.11.015

Cite this

@article{056aabe0fbca48ce972a9f442875cd42,

title = "Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations",

abstract = "The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.",

keywords = "Clara cell, Microarray, Nissl stain, Real-time Polymerase chain reaction",

author = "Tomoko Betsuyaku and Senior, {Robert M.}",

note = "Funding Information: The work was supported by the Respiratory Failure Research Group of the Ministry of Health, Labor, and Welfare, Japan, scientific research grant no. 14570532 from the Ministry of Education, Science, Culture and Sports, Japan to TB and grant nos HL 29594 and HL 47328 from NHLBI, National Institutes of Health and the Alan A. and Edith L. Wolff Charitable Trust to RMS. We thank Dr. Mark A. Watson and the technical staff of the Siteman Cancer Center GeneChip Facility at Washington University Medical Center for advice and assistance.",

year = "2004",

month = jun,

doi = "10.1016/j.micron.2003.11.015",

language = "English",

volume = "35",

pages = "229--234",

journal = "Micron and Microscopica Acta",

issn = "0968-4328",

publisher = "Elsevier Limited",

number = "4",

}

TY - JOUR

T1 - Laser capture microdissection and mRNA characterization of mouse airway epithelium

T2 - Methodological considerations

AU - Betsuyaku, Tomoko

AU - Senior, Robert M.

N1 - Funding Information: The work was supported by the Respiratory Failure Research Group of the Ministry of Health, Labor, and Welfare, Japan, scientific research grant no. 14570532 from the Ministry of Education, Science, Culture and Sports, Japan to TB and grant nos HL 29594 and HL 47328 from NHLBI, National Institutes of Health and the Alan A. and Edith L. Wolff Charitable Trust to RMS. We thank Dr. Mark A. Watson and the technical staff of the Siteman Cancer Center GeneChip Facility at Washington University Medical Center for advice and assistance.

PY - 2004/6

Y1 - 2004/6

N2 - The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.

AB - The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.

KW - Clara cell

KW - Microarray

KW - Nissl stain

KW - Real-time Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=1542350621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542350621&partnerID=8YFLogxK

U2 - 10.1016/j.micron.2003.11.015

DO - 10.1016/j.micron.2003.11.015

M3 - Article

C2 - 15003609

AN - SCOPUS:1542350621

SN - 0968-4328

VL - 35

SP - 229

EP - 234

JO - Micron and Microscopica Acta

JF - Micron and Microscopica Acta

IS - 4

ER -

Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this