Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa; Risa Otsuka; Shunichi Sato; Yasushi Satoh; Kunio Takishima; Hiroshi Ashida; Hideyuki Okano; Minoru Obara

doi:10.1143/JJAP.46.L1243

Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa, Risa Otsuka, Shunichi Sato, Yasushi Satoh, Kunio Takishima, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Department of Physiology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

Original language	English
Pages (from-to)	L1243-L1245
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	46
Issue number	45-49
DOIs	https://doi.org/10.1143/JJAP.46.L1243
Publication status	Published - 2007 Dec 14

Keywords

Cationic liposome
Gene delivery
Gene therapy
Laser
Nonviral vectors

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy (miscellaneous)
Physics and Astronomy(all)

Access to Document

10.1143/JJAP.46.L1243

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Laser-induced stress wave-assisted gene transfection with cationic liposome. / Terakawa, Mitsuhiro; Otsuka, Risa; Sato, Shunichi; Satoh, Yasushi; Takishima, Kunio; Ashida, Hiroshi; Okano, Hideyuki; Obara, Minoru.

In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 46, No. 45-49, 14.12.2007, p. L1243-L1245.

Research output: Contribution to journal › Article › peer-review

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