Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa, Risa Otsuka, Shunichi Sato, Yasushi Satoh, Kunio Takishima, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

Original languageEnglish
Pages (from-to)L1243-L1245
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume46
Issue number45-49
DOIs
Publication statusPublished - 2007 Dec 14

Keywords

  • Cationic liposome
  • Gene delivery
  • Gene therapy
  • Laser
  • Nonviral vectors

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy (miscellaneous)
  • Physics and Astronomy(all)

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