Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa, Risa Otsuka, Shunichi Sato, Yasushi Satoh, Kunio Takishima, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume46
Issue number45-49
DOIs
Publication statusPublished - 2007 Dec 14

Fingerprint

stress waves
Liposomes
genes
Genes
laser targets
plasmids
Lasers
cells
lasers
DNA
deoxyribonucleic acid
Gene transfer
Laser pulses
Irradiation
irradiation
pulses

Keywords

  • Cationic liposome
  • Gene delivery
  • Gene therapy
  • Laser
  • Nonviral vectors

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Laser-induced stress wave-assisted gene transfection with cationic liposome. / Terakawa, Mitsuhiro; Otsuka, Risa; Sato, Shunichi; Satoh, Yasushi; Takishima, Kunio; Ashida, Hiroshi; Okano, Hideyuki; Obara, Minoru.

In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 46, No. 45-49, 14.12.2007.

Research output: Contribution to journalArticle

Terakawa, Mitsuhiro ; Otsuka, Risa ; Sato, Shunichi ; Satoh, Yasushi ; Takishima, Kunio ; Ashida, Hiroshi ; Okano, Hideyuki ; Obara, Minoru. / Laser-induced stress wave-assisted gene transfection with cationic liposome. In: Japanese Journal of Applied Physics, Part 2: Letters. 2007 ; Vol. 46, No. 45-49.
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