Abstract
We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.
| Original language | English |
|---|---|
| Pages (from-to) | L1243-L1245 |
| Journal | Japanese Journal of Applied Physics, Part 2: Letters |
| Volume | 46 |
| Issue number | 45-49 |
| DOIs | |
| Publication status | Published - 2007 Dec 14 |
Keywords
- Cationic liposome
- Gene delivery
- Gene therapy
- Laser
- Nonviral vectors
ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy (miscellaneous)
- Physics and Astronomy(all)
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Cite this
Terakawa, M., Otsuka, R., Sato, S., Satoh, Y., Takishima, K., Ashida, H., Okano, H., & Obara, M. (2007). Laser-induced stress wave-assisted gene transfection with cationic liposome. Japanese Journal of Applied Physics, Part 2: Letters, 46(45-49), L1243-L1245. https://doi.org/10.1143/JJAP.46.L1243