Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa; Risa Otsuka; Shunichi Sato; Yasushi Satoh; Kunio Takishima; Hiroshi Ashida; Hideyuki Okano; Minoru Obara

doi:10.1143/JJAP.46.L1243

Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa, Risa Otsuka, Shunichi Sato, Yasushi Satoh, Kunio Takishima, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

Original language	English
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	46
Issue number	45-49
DOIs	https://doi.org/10.1143/JJAP.46.L1243
Publication status	Published - 2007 Dec 14

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Keywords

Cationic liposome
Gene delivery
Gene therapy
Laser
Nonviral vectors

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

Cite this

Terakawa, M., Otsuka, R., Sato, S., Satoh, Y., Takishima, K., Ashida, H., Okano, H., & Obara, M. (2007). Laser-induced stress wave-assisted gene transfection with cationic liposome. Japanese Journal of Applied Physics, Part 2: Letters, 46(45-49). https://doi.org/10.1143/JJAP.46.L1243

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AU - Sato, Shunichi

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AU - Ashida, Hiroshi

AU - Okano, Hideyuki

AU - Obara, Minoru

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Access to Document

10.1143/JJAP.46.L1243