Abstract
We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.
Original language | English |
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Pages (from-to) | L1243-L1245 |
Journal | Japanese Journal of Applied Physics, Part 2: Letters |
Volume | 46 |
Issue number | 45-49 |
DOIs | |
Publication status | Published - 2007 Dec 14 |
Keywords
- Cationic liposome
- Gene delivery
- Gene therapy
- Laser
- Nonviral vectors
ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy(all)