Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa; Risa Otsuka; Shunichi Sato; Yasushi Satoh; Kunio Takishima; Hiroshi Ashida; Hideyuki Okano; Minoru Obara

doi:10.1143/JJAP.46.L1243

Laser-induced stress wave-assisted gene transfection with cationic liposome

Mitsuhiro Terakawa, Risa Otsuka, Shunichi Sato, Yasushi Satoh, Kunio Takishima, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

Original language	English
Pages (from-to)	L1243-L1245
Journal	Japanese Journal of Applied Physics, Part 2: Letters
Volume	46
Issue number	45-49
DOIs	https://doi.org/10.1143/JJAP.46.L1243
Publication status	Published - 2007 Dec 14

Keywords

Cationic liposome
Gene delivery
Gene therapy
Laser
Nonviral vectors

ASJC Scopus subject areas

Engineering(all)
Physics and Astronomy(all)

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10.1143/JJAP.46.L1243

Cite this

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title = "Laser-induced stress wave-assisted gene transfection with cationic liposome",

abstract = "We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.",

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doi = "10.1143/JJAP.46.L1243",

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T1 - Laser-induced stress wave-assisted gene transfection with cationic liposome

AU - Terakawa, Mitsuhiro

AU - Otsuka, Risa

AU - Sato, Shunichi

AU - Satoh, Yasushi

AU - Takishima, Kunio

AU - Ashida, Hiroshi

AU - Okano, Hideyuki

AU - Obara, Minoru

PY - 2007/12/14

Y1 - 2007/12/14

N2 - We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

AB - We showed that efficiency of gene transfer using laser-induced stress waves (LISWs) can be drastically enhanced by modifying plasmid DNA with cationic liposome. Cultured NIH 3T3 cells were covered with a suspension including plasmid DNA that had been modified with Lipofectamine, and a laser target was placed over the suspension. The cells were exposed to stress waves generated by irradiation of the laser target with 6-ns laser pulses. The cells showed a significantly higher transfection efficiency compared with that of cells treated with LISWs alone or Lipofectamine alone.

KW - Cationic liposome

KW - Gene delivery

KW - Gene therapy

KW - Laser

KW - Nonviral vectors

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Laser-induced stress wave-assisted gene transfection with cationic liposome

Abstract

Keywords

ASJC Scopus subject areas

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