Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex

Harumi Hotta, Kazuto Masamoto, Sae Uchida, Yuta Sekiguchi, Hiroyuki Takuwa, Hiroshi Kawaguchi, Kazuhiro Shigemoto, Ryo Sudo, Kazuo Tanishita, Hiroshi Ito, Iwao Kanno

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To clarify mechanisms through which activation of the nucleus basalis of Meynert (NBM) increases cerebral cortical blood flow, we examined whether cortical parenchymal arteries dilate during NBM stimulation in anesthetized mice. We used two-photon microscopy to measure the diameter of single penetrating arteries at different depths (∼800 μm, layers I to V) of the frontal cortex, and examined changes in the diameter during focal electrical stimulation of the NBM (0.5 ms at 30 to 50 μA and 50 Hz) and hypercapnia (3% CO 2 inhalation). Stimulation of the NBM caused diameter of penetrating arteries to increase by 9% to 13% of the prestimulus diameter throughout the different layers of the cortex, except at the cortical surface and upper part of layer V, where the diameter of penetrating arteries increased only slightly during NBM stimulation. Hypercapnia caused obvious dilation of the penetrating arteries in all cortical layers, including the surface arteries. The diameters began to increase within 1 second after the onset of NBM stimulation in the upper cortical layers, and later in lower layers. Our results indicate that activation of the NBM dilates cortical penetrating arteries in a layer-specific manner in magnitude and latency, presumably related to the density of cholinergic nerve terminals from the NBM.

Original languageEnglish
Pages (from-to)1440-1447
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Volume33
Issue number9
DOIs
Publication statusPublished - 2013 Sep

Fingerprint

Basal Nucleus of Meynert
Frontal Lobe
Dilatation
Arteries
Hypercapnia
Cerebrovascular Circulation
Carbon Monoxide
Photons
Cholinergic Agents
Inhalation
Electric Stimulation
Microscopy

Keywords

  • cerebral cortex
  • diameter
  • nucleus basalis of Meynert
  • penetrating artery
  • two-photon microscopy
  • vasodilation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex. / Hotta, Harumi; Masamoto, Kazuto; Uchida, Sae; Sekiguchi, Yuta; Takuwa, Hiroyuki; Kawaguchi, Hiroshi; Shigemoto, Kazuhiro; Sudo, Ryo; Tanishita, Kazuo; Ito, Hiroshi; Kanno, Iwao.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 33, No. 9, 09.2013, p. 1440-1447.

Research output: Contribution to journalArticle

Hotta, H, Masamoto, K, Uchida, S, Sekiguchi, Y, Takuwa, H, Kawaguchi, H, Shigemoto, K, Sudo, R, Tanishita, K, Ito, H & Kanno, I 2013, 'Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex', Journal of Cerebral Blood Flow and Metabolism, vol. 33, no. 9, pp. 1440-1447. https://doi.org/10.1038/jcbfm.2013.92
Hotta, Harumi ; Masamoto, Kazuto ; Uchida, Sae ; Sekiguchi, Yuta ; Takuwa, Hiroyuki ; Kawaguchi, Hiroshi ; Shigemoto, Kazuhiro ; Sudo, Ryo ; Tanishita, Kazuo ; Ito, Hiroshi ; Kanno, Iwao. / Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex. In: Journal of Cerebral Blood Flow and Metabolism. 2013 ; Vol. 33, No. 9. pp. 1440-1447.
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