LC-MS-based quantification method for Achyranthes root saponins

Yuki Kawahara, Tatsuro Hoshino, Hidetaka Morimoto, Tomofumi Shinizu, Yuuji Narukawa, Hiroyuki Fuchino, Nobuo Kawahara, Fumiyuki Kiuchi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A liquid chromatography mass spectrometry (LC-MS) method was developed for simultaneous quantitative analysis of Achyranthes root saponins: chikusetsusaponins IVa (1) and V (2), achyranthosides B (3), C (4), D (5), E (6), and G (7), sulfachyranthosides B (8) and D (9), and betavulgarosides II (10) and IV (11). Satisfactory separation of the saponins was achieved with the use of a volatile ion-pair reagent (dihexyl ammonium acetate) on a phenyl-hexylated silica gel column, and the amounts of saponins extracted under three different conditions were determined. When Achyranthes root was extracted with water at room temperature, achyranthosides B (3) and D (5) were the major saponins, and smaller amounts of other saponins (4, 6-11) were present. However, the amounts of chikusetsusaponins (1 and 2) were negligible. Under the condition to make a standard decoction of a Kampo formula, the major saponins were achyranthosides B (3), C (4), and D (5), and small amounts of chikusetsusaponins IVa (1) and V (2) appeared, whereas prolonged heating largely increased the amounts of chikusetsusaponins. This method can be used for quality control of Achyranthes root.

Original languageEnglish
Pages (from-to)102-106
Number of pages5
JournalJournal of Natural Medicines
Volume70
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Achyranthes
Saponins
Liquid chromatography
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Kampo Medicine
Silica Gel
Quality Control
Heating
Quality control
Ions
Temperature
Water
Chemical analysis

Keywords

  • Achyranthes bidentata
  • Achyranthoside
  • LC-MS
  • Oleanan saponin
  • Quantitative analysis
  • Sulfachyranthoside

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

Cite this

LC-MS-based quantification method for Achyranthes root saponins. / Kawahara, Yuki; Hoshino, Tatsuro; Morimoto, Hidetaka; Shinizu, Tomofumi; Narukawa, Yuuji; Fuchino, Hiroyuki; Kawahara, Nobuo; Kiuchi, Fumiyuki.

In: Journal of Natural Medicines, Vol. 70, No. 1, 01.01.2016, p. 102-106.

Research output: Contribution to journalArticle

Kawahara, Y, Hoshino, T, Morimoto, H, Shinizu, T, Narukawa, Y, Fuchino, H, Kawahara, N & Kiuchi, F 2016, 'LC-MS-based quantification method for Achyranthes root saponins', Journal of Natural Medicines, vol. 70, no. 1, pp. 102-106. https://doi.org/10.1007/s11418-015-0942-4
Kawahara, Yuki ; Hoshino, Tatsuro ; Morimoto, Hidetaka ; Shinizu, Tomofumi ; Narukawa, Yuuji ; Fuchino, Hiroyuki ; Kawahara, Nobuo ; Kiuchi, Fumiyuki. / LC-MS-based quantification method for Achyranthes root saponins. In: Journal of Natural Medicines. 2016 ; Vol. 70, No. 1. pp. 102-106.
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AB - A liquid chromatography mass spectrometry (LC-MS) method was developed for simultaneous quantitative analysis of Achyranthes root saponins: chikusetsusaponins IVa (1) and V (2), achyranthosides B (3), C (4), D (5), E (6), and G (7), sulfachyranthosides B (8) and D (9), and betavulgarosides II (10) and IV (11). Satisfactory separation of the saponins was achieved with the use of a volatile ion-pair reagent (dihexyl ammonium acetate) on a phenyl-hexylated silica gel column, and the amounts of saponins extracted under three different conditions were determined. When Achyranthes root was extracted with water at room temperature, achyranthosides B (3) and D (5) were the major saponins, and smaller amounts of other saponins (4, 6-11) were present. However, the amounts of chikusetsusaponins (1 and 2) were negligible. Under the condition to make a standard decoction of a Kampo formula, the major saponins were achyranthosides B (3), C (4), and D (5), and small amounts of chikusetsusaponins IVa (1) and V (2) appeared, whereas prolonged heating largely increased the amounts of chikusetsusaponins. This method can be used for quality control of Achyranthes root.

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