LC-MS-based quantification method for Achyranthes root saponins

Yuki Kawahara, Tatsuro Hoshino, Hidetaka Morimoto, Tomofumi Shinizu, Yuji Narukawa, Hiroyuki Fuchino, Nobuo Kawahara, Fumiyuki Kiuchi

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


A liquid chromatography mass spectrometry (LC-MS) method was developed for simultaneous quantitative analysis of Achyranthes root saponins: chikusetsusaponins IVa (1) and V (2), achyranthosides B (3), C (4), D (5), E (6), and G (7), sulfachyranthosides B (8) and D (9), and betavulgarosides II (10) and IV (11). Satisfactory separation of the saponins was achieved with the use of a volatile ion-pair reagent (dihexyl ammonium acetate) on a phenyl-hexylated silica gel column, and the amounts of saponins extracted under three different conditions were determined. When Achyranthes root was extracted with water at room temperature, achyranthosides B (3) and D (5) were the major saponins, and smaller amounts of other saponins (4, 6-11) were present. However, the amounts of chikusetsusaponins (1 and 2) were negligible. Under the condition to make a standard decoction of a Kampo formula, the major saponins were achyranthosides B (3), C (4), and D (5), and small amounts of chikusetsusaponins IVa (1) and V (2) appeared, whereas prolonged heating largely increased the amounts of chikusetsusaponins. This method can be used for quality control of Achyranthes root.

Original languageEnglish
Pages (from-to)102-106
Number of pages5
JournalJournal of Natural Medicines
Issue number1
Publication statusPublished - 2016 Jan 1


  • Achyranthes bidentata
  • Achyranthoside
  • LC-MS
  • Oleanan saponin
  • Quantitative analysis
  • Sulfachyranthoside

ASJC Scopus subject areas

  • Molecular Medicine


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