Lecithinized brain-derived neurotrophic factor promotes the differentiation of embryonic stem cells in vitro and in vivo

Aki Kitagawa, Toshiaki Nakayama, Mitsuko Takenaga, Kayo Matsumoto, Yukie Tokura, Yuki Ohta, Manabu Ichinohe, Yoko Yamaguchi, Noboru Suzuki, Hideyuki Okano, Rie Igarashi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The addition of lecithin molecules to brain-derived neurotrophic factor (BDNF) has been reported to markedly enhance its pharmacological effect in vivo. In the current study, we show that lecithinized BDNF (PC-BDNF) has a higher affinity than BDNF for neural precursor cells. Although BDNF only slightly increased the expression of the genes for Mash-1, p35, 68 kDa neurofilament, and TrkB receptor, PC-BDNF caused a significant increase in their expression. PC-BDNF also increased the level of neurofilament protein and dramatically increased TrkB mRNA gene expression, which was followed by a sustained activation of the p42/p44 extracellular-regulated kinases. Finally, transplantation of PC-BDNF-treated cells was more effective than BDNF-treated cells at improving impaired motor function caused by spinal cord injury. These findings showed that PC-BDNF has a better potential than BDNF for promoting neural differentiation, partly due to a higher cellular affinity. Furthermore, PC-BDNF-treated cells could be useful for transplantation therapy for central nervous system injuries.

Original languageEnglish
Pages (from-to)1051-1057
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number4
DOIs
Publication statusPublished - 2005 Mar 25

Keywords

  • BDNF
  • Differentiation
  • Embryonic stem cells
  • Lecithinized-BDNF
  • Spinal cord injury

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Lecithinized brain-derived neurotrophic factor promotes the differentiation of embryonic stem cells in vitro and in vivo'. Together they form a unique fingerprint.

  • Cite this

    Kitagawa, A., Nakayama, T., Takenaga, M., Matsumoto, K., Tokura, Y., Ohta, Y., Ichinohe, M., Yamaguchi, Y., Suzuki, N., Okano, H., & Igarashi, R. (2005). Lecithinized brain-derived neurotrophic factor promotes the differentiation of embryonic stem cells in vitro and in vivo. Biochemical and Biophysical Research Communications, 328(4), 1051-1057. https://doi.org/10.1016/j.bbrc.2005.01.063