TY - JOUR
T1 - Lecithinized brain-derived neurotrophic factor promotes the differentiation of embryonic stem cells in vitro and in vivo
AU - Kitagawa, Aki
AU - Nakayama, Toshiaki
AU - Takenaga, Mitsuko
AU - Matsumoto, Kayo
AU - Tokura, Yukie
AU - Ohta, Yuki
AU - Ichinohe, Manabu
AU - Yamaguchi, Yoko
AU - Suzuki, Noboru
AU - Okano, Hideyuki
AU - Igarashi, Rie
N1 - Funding Information:
This study was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2005/3/25
Y1 - 2005/3/25
N2 - The addition of lecithin molecules to brain-derived neurotrophic factor (BDNF) has been reported to markedly enhance its pharmacological effect in vivo. In the current study, we show that lecithinized BDNF (PC-BDNF) has a higher affinity than BDNF for neural precursor cells. Although BDNF only slightly increased the expression of the genes for Mash-1, p35, 68 kDa neurofilament, and TrkB receptor, PC-BDNF caused a significant increase in their expression. PC-BDNF also increased the level of neurofilament protein and dramatically increased TrkB mRNA gene expression, which was followed by a sustained activation of the p42/p44 extracellular-regulated kinases. Finally, transplantation of PC-BDNF-treated cells was more effective than BDNF-treated cells at improving impaired motor function caused by spinal cord injury. These findings showed that PC-BDNF has a better potential than BDNF for promoting neural differentiation, partly due to a higher cellular affinity. Furthermore, PC-BDNF-treated cells could be useful for transplantation therapy for central nervous system injuries.
AB - The addition of lecithin molecules to brain-derived neurotrophic factor (BDNF) has been reported to markedly enhance its pharmacological effect in vivo. In the current study, we show that lecithinized BDNF (PC-BDNF) has a higher affinity than BDNF for neural precursor cells. Although BDNF only slightly increased the expression of the genes for Mash-1, p35, 68 kDa neurofilament, and TrkB receptor, PC-BDNF caused a significant increase in their expression. PC-BDNF also increased the level of neurofilament protein and dramatically increased TrkB mRNA gene expression, which was followed by a sustained activation of the p42/p44 extracellular-regulated kinases. Finally, transplantation of PC-BDNF-treated cells was more effective than BDNF-treated cells at improving impaired motor function caused by spinal cord injury. These findings showed that PC-BDNF has a better potential than BDNF for promoting neural differentiation, partly due to a higher cellular affinity. Furthermore, PC-BDNF-treated cells could be useful for transplantation therapy for central nervous system injuries.
KW - BDNF
KW - Differentiation
KW - Embryonic stem cells
KW - Lecithinized-BDNF
KW - Spinal cord injury
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U2 - 10.1016/j.bbrc.2005.01.063
DO - 10.1016/j.bbrc.2005.01.063
M3 - Article
C2 - 15707984
AN - SCOPUS:13744253430
SN - 0006-291X
VL - 328
SP - 1051
EP - 1057
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -