Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling

Hiroko Shimazaki, Kozue Saito, Atsushi Matsuda, Kazumi Sawakami, Minoru Kariya, Osamu Segawa, Yukiko Miyashita, Tetsuya Ueda, Michinori Koizuka, Kazuhiro Nakamura, Hiroyuki Kaji, Hideji Tajima, Atsushi Kuno

Research output: Contribution to journalArticle

Abstract

A quantitative description of glyco-alteration/differences in diseases can lead to the development of a diagnostic agent for use in vitro to monitor the degree of change in target glycoproteins. Analytical systems have been developed along with the progress of omics-oriented technologies. For clinical implementation, their full automation is required with an apparatus that is simple to operate. Here, we report an automatic analysis system for quantitative characterization of glyco-alteration/differences that depends on the unique strategy of "bead arrays in a single tip." The alternative lectin array can obtain a minimum characterization of the glycan profile for nanogram quantities of an endogenous glycoprotein. A simple autopipetting robot produces the precise chemiluminescence detection of glycan-lectin interactions with a wide dynamic range that is superior to fluorescence-based lectin arrays. The tip-based array format enables automatic glycan profiling from sample pretreatment to detection with low variation and linear detection, which may facilitate the use of this lectin array in clinical practice.

Original languageEnglish
Pages (from-to)11162-11169
Number of pages8
JournalAnalytical chemistry
Volume91
Issue number17
DOIs
Publication statusPublished - 2019 Sep 3

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Lectins
Glycoproteins
Polysaccharides
Chemiluminescence
Automation
Fluorescence
Robots

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Shimazaki, H., Saito, K., Matsuda, A., Sawakami, K., Kariya, M., Segawa, O., ... Kuno, A. (2019). Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling. Analytical chemistry, 91(17), 11162-11169. https://doi.org/10.1021/acs.analchem.9b01876

Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling. / Shimazaki, Hiroko; Saito, Kozue; Matsuda, Atsushi; Sawakami, Kazumi; Kariya, Minoru; Segawa, Osamu; Miyashita, Yukiko; Ueda, Tetsuya; Koizuka, Michinori; Nakamura, Kazuhiro; Kaji, Hiroyuki; Tajima, Hideji; Kuno, Atsushi.

In: Analytical chemistry, Vol. 91, No. 17, 03.09.2019, p. 11162-11169.

Research output: Contribution to journalArticle

Shimazaki, H, Saito, K, Matsuda, A, Sawakami, K, Kariya, M, Segawa, O, Miyashita, Y, Ueda, T, Koizuka, M, Nakamura, K, Kaji, H, Tajima, H & Kuno, A 2019, 'Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling', Analytical chemistry, vol. 91, no. 17, pp. 11162-11169. https://doi.org/10.1021/acs.analchem.9b01876
Shimazaki, Hiroko ; Saito, Kozue ; Matsuda, Atsushi ; Sawakami, Kazumi ; Kariya, Minoru ; Segawa, Osamu ; Miyashita, Yukiko ; Ueda, Tetsuya ; Koizuka, Michinori ; Nakamura, Kazuhiro ; Kaji, Hiroyuki ; Tajima, Hideji ; Kuno, Atsushi. / Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling. In: Analytical chemistry. 2019 ; Vol. 91, No. 17. pp. 11162-11169.
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