Leucine-rich repeat-containing G-protein-coupled receptor 5 expression and clinicopathological features of colorectal neuroendocrine neoplasms

Tomoyuki Nakajima, Takeshi Uehara, Yukihiro Kobayashi, Yasuhiro Kinugawa, Kazuhiro Yamanoi, Yasuhiro Maruyama, Tomoaki Suga, Hiroyoshi Ota

Research output: Contribution to journalArticle

Abstract

LGR5 is expressed in various tumors and has been identified as a putative intestinal stem cell marker. Here we investigated LGR5 expression in colorectal neuroendocrine neoplasms and analyzed the correlation with pathological characteristics. We evaluated the clinicopathological features of 8 neuroendocrine tumor (NET) grade 1 (NET G1), 4 NET Grade 2 (NET G2), and 8 NET Grade 3 (NET G3; also termed neuroendocrine carcinoma, or NEC) cases. We examined LGR5 expression using an RNAscope, a newly developed RNA in situ hybridization technique, with a tissue microarray of the neuroendocrine neoplasm samples. LGR5 staining in individual tumor cells was semi-quantitatively scored using an H-score scale. We also performed a combination of LGR5 RNA in situ hybridization and synaptophysin immunohistochemistry. All cases contained tumor cells with some LGR5-positive dots. For all cases, H-scores showed a positive correlation with nuclear beta-catenin expression. In the NEC group, there was a strong positive correlation between H-score and beta-catenin expression. Our findings suggest that LGR5 may serve as a stem cell marker in NEC, as is the case in colon adenocarcinoma. The positive correlation between H-score and beta-catenin expression suggests that LGR5 expression might be affected by beta-catenin expression in neuroendocrine neoplasms and especially in NEC.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalPathology international
Volume68
Issue number8
DOIs
Publication statusPublished - 2018 Aug 1
Externally publishedYes

Fingerprint

G-Protein-Coupled Receptors
Leucine
Neuroendocrine Tumors
beta Catenin
Colorectal Neoplasms
Neoplasms
In Situ Hybridization
Stem Cells
RNA
Neuroendocrine Carcinoma
Synaptophysin
Colon
Adenocarcinoma
Immunohistochemistry
Staining and Labeling

Keywords

  • colorectal neuroendocrine neoplasms
  • Leucine-rich repeat-containing G-protein-coupled receptor 5
  • RNA in situ hybridization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Leucine-rich repeat-containing G-protein-coupled receptor 5 expression and clinicopathological features of colorectal neuroendocrine neoplasms. / Nakajima, Tomoyuki; Uehara, Takeshi; Kobayashi, Yukihiro; Kinugawa, Yasuhiro; Yamanoi, Kazuhiro; Maruyama, Yasuhiro; Suga, Tomoaki; Ota, Hiroyoshi.

In: Pathology international, Vol. 68, No. 8, 01.08.2018, p. 467-472.

Research output: Contribution to journalArticle

Nakajima, Tomoyuki ; Uehara, Takeshi ; Kobayashi, Yukihiro ; Kinugawa, Yasuhiro ; Yamanoi, Kazuhiro ; Maruyama, Yasuhiro ; Suga, Tomoaki ; Ota, Hiroyoshi. / Leucine-rich repeat-containing G-protein-coupled receptor 5 expression and clinicopathological features of colorectal neuroendocrine neoplasms. In: Pathology international. 2018 ; Vol. 68, No. 8. pp. 467-472.
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