Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF

T. Inukai, K. Sugita, K. Iijima, K. Goi, T. Tezuka, S. Kojika, K. Kagami, Takehiko Mori, A. Kinoshita, T. Suzuki, T. O-Koyama, S. Nakazawa

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Abstract

We report a 20-month-old boy with acute lymphoblastic leukemia with the 11q23 translocation whose blasts markedly increased in peripheral blood after intravenous granulocyte colony-stimulating factor (G-CSF) administration, but disappeared after stopping G-CSF. The in vitro study showed that the leukemic cells separated from this patient expressed G-CSF receptor (G-CSFR) and an addition of G-CSF stimulated their proliferation by 3H-thymidine incorporation assay (stimulation index, 4.9). To clarify whether or not leukemic cells with 11q23 translocations generally express G-CSFR and show proliferative response to G-CSF, we performed the similar in vitro experiments using eight leukemic cell lines with 11q23 translocations. We found that all cell lines examined expressed G-CSFR (20-98%) and proliferation of seven leukemic cell lines was significantly enhanced in response to G-CSF (stimulation index > 1.5 in five cell lines), suggesting a possible participation of the G-CSF/G-CSFR interaction in the process of growth regulation of leukemic cells with 11q23 translocations.

Original languageEnglish
Pages (from-to)382-389
Number of pages8
JournalLeukemia
Volume12
Issue number3
DOIs
Publication statusPublished - 1998

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Granulocyte Colony-Stimulating Factor Receptors
Granulocyte Colony-Stimulating Factor
Cell Line
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Thymidine
Growth

Keywords

  • 11q23 translocation
  • G-CSF
  • G-CSFR
  • Infant leukemia

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF. / Inukai, T.; Sugita, K.; Iijima, K.; Goi, K.; Tezuka, T.; Kojika, S.; Kagami, K.; Mori, Takehiko; Kinoshita, A.; Suzuki, T.; O-Koyama, T.; Nakazawa, S.

In: Leukemia, Vol. 12, No. 3, 1998, p. 382-389.

Research output: Contribution to journalArticle

Inukai, T, Sugita, K, Iijima, K, Goi, K, Tezuka, T, Kojika, S, Kagami, K, Mori, T, Kinoshita, A, Suzuki, T, O-Koyama, T & Nakazawa, S 1998, 'Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF', Leukemia, vol. 12, no. 3, pp. 382-389. https://doi.org/10.1038/sj.leu.2400951
Inukai, T. ; Sugita, K. ; Iijima, K. ; Goi, K. ; Tezuka, T. ; Kojika, S. ; Kagami, K. ; Mori, Takehiko ; Kinoshita, A. ; Suzuki, T. ; O-Koyama, T. ; Nakazawa, S. / Leukemic cells with 11q23 translocations express granulocyte colony-stimulating factor (G-CSF) receptor and their proliferation is stimulated with G-CSF. In: Leukemia. 1998 ; Vol. 12, No. 3. pp. 382-389.
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