Licochalcones suppress degranulation by decreasing the intracellular Ca2+ level and tyrosine phosphorylation of ERK in RBL-2H3 cells

Shota Tanifuji, Eriko Aizu-Yokota, Megumi Funakoshi-Tago, Yoshiko Sonoda, Hideo Inoue, Tadashi Kasahara

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mast cells play a key role in allergic inflammation by releasing various mediators, such as histamine, serotonin, leukotrienes and cytokines. A signaling cascade of events activated by stimulation with antigens contributes to the regulation of mast cell degranulation. While various anti-inflammatory and anti-allergic drugs have been developed that inhibit degranulation of mast cells, the inhibitory mechanism has been poorly understood. Licochalcone A (Lico A) is a retrochalcone isolated from the root of Xinjiang liquorice and has been reported to exhibit various biological activities such as anti-inflammatory activity. We examined the effects of Lico A and related chalcones on degranulation in a rat basophilic leukemia cell line, RBL-2H3. Whereas Lico A and licochalcone C (Lico C) exhibited inhibitory activity with cytotoxicity, licochalcone D (Lico D) significantly inhibited the degranulation in RBL-2H3 cells with low cytotoxicity. Moreover, Lico D significantly inhibited the Ca2+ influx and phosphorylation of extracellular signal regulated kinase (ERK) and MEK. These results suggest that Lico D inhibits mast cell degranulation via the inhibition of both extracellular Ca2+ influx and activation of the MEK-ERK pathway.

Original languageEnglish
Pages (from-to)769-776
Number of pages8
JournalInternational Immunopharmacology
Volume10
Issue number7
DOIs
Publication statusPublished - 2010 Jul 1

Keywords

  • Ca influx
  • Degranulation
  • ERK
  • Licochalcone D
  • Mast cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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