Light-dark condition regulates sirtuin mRNA levels in the retina

Norimitsu Ban, Yoko Ozawa, Takaaki Inaba, Seiji Miyake, Mitsuhiro Watanabe, Ken Shinmura, Kazuo Tsubota

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Sirtuins (Sirt1-7) are nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases/ADP-ribosyltransferases that modulate many metabolic responses affecting aging. Sirtuins expressed in tissues and organs involved in systemic metabolism have been extensively studied. However, the characteristics of sirtuins in the retina, where local energy expenditure changes dynamically in response to light stimuli, are largely unknown. Here we analyzed sirtuin mRNA levels by real-time PCR, and found that all seven sirtuins are highly expressed in the retina compared with other tissues, such as liver. We then analyzed the sirtuin mRNA profiles in the retina over time, under a 12-h light/12-h dark cycle (LD condition) and in constant darkness (DD condition). All seven sirtuins showed significant daily variation under the LD condition, with all except Sirt6 being increased in the dark phase. The expression patterns were different under the DD condition, suggesting that sirtuin mRNA levels except Sirt6 are affected by light-dark condition. These findings were not obtained in the brain and liver. In addition, the mRNA expression patterns of Nicotinamide phosphoribosyltransferase (Nampt), peroxisome proliferator-activated receptor gamma coactivator (PGC1α), and transcription factor A, mitochondrial (Tfam) in the retina, were similar to those of the sirtuins except Sirt6. Our observations provide new insights into the metabolic mechanisms of the retina and the sirtuins' regulatory systems.

Original languageEnglish
Pages (from-to)1212-1217
Number of pages6
JournalExperimental Gerontology
Volume48
Issue number11
DOIs
Publication statusPublished - 2013 Nov

Fingerprint

Sirtuins
Retina
Light
Messenger RNA
Liver
Nicotinamide Phosphoribosyltransferase
ADP Ribose Transferases
Tissue
PPAR gamma
Darkness
Metabolism
NAD
Energy Metabolism
Real-Time Polymerase Chain Reaction
Brain
Transcription Factors
Aging of materials

Keywords

  • Light
  • Metabolism
  • Mitochondria
  • Nampt
  • Retina
  • Sirtuins

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology

Cite this

Light-dark condition regulates sirtuin mRNA levels in the retina. / Ban, Norimitsu; Ozawa, Yoko; Inaba, Takaaki; Miyake, Seiji; Watanabe, Mitsuhiro; Shinmura, Ken; Tsubota, Kazuo.

In: Experimental Gerontology, Vol. 48, No. 11, 11.2013, p. 1212-1217.

Research output: Contribution to journalArticle

Ban, Norimitsu ; Ozawa, Yoko ; Inaba, Takaaki ; Miyake, Seiji ; Watanabe, Mitsuhiro ; Shinmura, Ken ; Tsubota, Kazuo. / Light-dark condition regulates sirtuin mRNA levels in the retina. In: Experimental Gerontology. 2013 ; Vol. 48, No. 11. pp. 1212-1217.
@article{8a9c22fe34794b9c9ab961d651aede8e,
title = "Light-dark condition regulates sirtuin mRNA levels in the retina",
abstract = "Sirtuins (Sirt1-7) are nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases/ADP-ribosyltransferases that modulate many metabolic responses affecting aging. Sirtuins expressed in tissues and organs involved in systemic metabolism have been extensively studied. However, the characteristics of sirtuins in the retina, where local energy expenditure changes dynamically in response to light stimuli, are largely unknown. Here we analyzed sirtuin mRNA levels by real-time PCR, and found that all seven sirtuins are highly expressed in the retina compared with other tissues, such as liver. We then analyzed the sirtuin mRNA profiles in the retina over time, under a 12-h light/12-h dark cycle (LD condition) and in constant darkness (DD condition). All seven sirtuins showed significant daily variation under the LD condition, with all except Sirt6 being increased in the dark phase. The expression patterns were different under the DD condition, suggesting that sirtuin mRNA levels except Sirt6 are affected by light-dark condition. These findings were not obtained in the brain and liver. In addition, the mRNA expression patterns of Nicotinamide phosphoribosyltransferase (Nampt), peroxisome proliferator-activated receptor gamma coactivator (PGC1α), and transcription factor A, mitochondrial (Tfam) in the retina, were similar to those of the sirtuins except Sirt6. Our observations provide new insights into the metabolic mechanisms of the retina and the sirtuins' regulatory systems.",
keywords = "Light, Metabolism, Mitochondria, Nampt, Retina, Sirtuins",
author = "Norimitsu Ban and Yoko Ozawa and Takaaki Inaba and Seiji Miyake and Mitsuhiro Watanabe and Ken Shinmura and Kazuo Tsubota",
year = "2013",
month = "11",
doi = "10.1016/j.exger.2013.04.010",
language = "English",
volume = "48",
pages = "1212--1217",
journal = "Experimental Gerontology",
issn = "0531-5565",
publisher = "Elsevier Inc.",
number = "11",

}

TY - JOUR

T1 - Light-dark condition regulates sirtuin mRNA levels in the retina

AU - Ban, Norimitsu

AU - Ozawa, Yoko

AU - Inaba, Takaaki

AU - Miyake, Seiji

AU - Watanabe, Mitsuhiro

AU - Shinmura, Ken

AU - Tsubota, Kazuo

PY - 2013/11

Y1 - 2013/11

N2 - Sirtuins (Sirt1-7) are nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases/ADP-ribosyltransferases that modulate many metabolic responses affecting aging. Sirtuins expressed in tissues and organs involved in systemic metabolism have been extensively studied. However, the characteristics of sirtuins in the retina, where local energy expenditure changes dynamically in response to light stimuli, are largely unknown. Here we analyzed sirtuin mRNA levels by real-time PCR, and found that all seven sirtuins are highly expressed in the retina compared with other tissues, such as liver. We then analyzed the sirtuin mRNA profiles in the retina over time, under a 12-h light/12-h dark cycle (LD condition) and in constant darkness (DD condition). All seven sirtuins showed significant daily variation under the LD condition, with all except Sirt6 being increased in the dark phase. The expression patterns were different under the DD condition, suggesting that sirtuin mRNA levels except Sirt6 are affected by light-dark condition. These findings were not obtained in the brain and liver. In addition, the mRNA expression patterns of Nicotinamide phosphoribosyltransferase (Nampt), peroxisome proliferator-activated receptor gamma coactivator (PGC1α), and transcription factor A, mitochondrial (Tfam) in the retina, were similar to those of the sirtuins except Sirt6. Our observations provide new insights into the metabolic mechanisms of the retina and the sirtuins' regulatory systems.

AB - Sirtuins (Sirt1-7) are nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases/ADP-ribosyltransferases that modulate many metabolic responses affecting aging. Sirtuins expressed in tissues and organs involved in systemic metabolism have been extensively studied. However, the characteristics of sirtuins in the retina, where local energy expenditure changes dynamically in response to light stimuli, are largely unknown. Here we analyzed sirtuin mRNA levels by real-time PCR, and found that all seven sirtuins are highly expressed in the retina compared with other tissues, such as liver. We then analyzed the sirtuin mRNA profiles in the retina over time, under a 12-h light/12-h dark cycle (LD condition) and in constant darkness (DD condition). All seven sirtuins showed significant daily variation under the LD condition, with all except Sirt6 being increased in the dark phase. The expression patterns were different under the DD condition, suggesting that sirtuin mRNA levels except Sirt6 are affected by light-dark condition. These findings were not obtained in the brain and liver. In addition, the mRNA expression patterns of Nicotinamide phosphoribosyltransferase (Nampt), peroxisome proliferator-activated receptor gamma coactivator (PGC1α), and transcription factor A, mitochondrial (Tfam) in the retina, were similar to those of the sirtuins except Sirt6. Our observations provide new insights into the metabolic mechanisms of the retina and the sirtuins' regulatory systems.

KW - Light

KW - Metabolism

KW - Mitochondria

KW - Nampt

KW - Retina

KW - Sirtuins

UR - http://www.scopus.com/inward/record.url?scp=84883652690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883652690&partnerID=8YFLogxK

U2 - 10.1016/j.exger.2013.04.010

DO - 10.1016/j.exger.2013.04.010

M3 - Article

C2 - 23648587

AN - SCOPUS:84883652690

VL - 48

SP - 1212

EP - 1217

JO - Experimental Gerontology

JF - Experimental Gerontology

SN - 0531-5565

IS - 11

ER -