LINE1 family member is negative regulator of HLA-G expression

Masashi Ikeno, Nobutaka Suzuki, Megumi Kamiya, Yuji Takahashi, Jun Kudo, Tsuneko Okazaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Class Ia molecules of human leucocyte antigen (HLA-A,-B and-C) are widely expressed and play a central role in the immune system by presenting peptides derived from the lumen of the endoplasmic reticulum. In contrast, class Ib molecules such as HLA-G serve novel functions. The distribution of HLA-G is mostly limited to foetal trophoblastic tissues and some tumour tissues. The mechanism required for the tissue-specific regulation of the HLA-G gene has not been well understood. Here, we investigated the genomic regulation of HLA-G by manipulating one copy of a genomic DNA fragment on a human artificial chromosome. We identified a potential negative regulator of gene expression in a sequence upstream of HLA-G that overlapped with the long interspersed element (LINE1); silencing of HLA-G involved a DNA secondary structure generated in LINE1. The presence of a LINE1 gene silencer may explain the limited expression of HLA-G compared with other class I genes.

Original languageEnglish
Pages (from-to)10742-10752
Number of pages11
JournalNucleic Acids Research
Volume40
Issue number21
DOIs
Publication statusPublished - 2012 Nov

Fingerprint

HLA-G Antigens
Human Artificial Chromosomes
MHC Class I Genes
HLA-A Antigens
HLA-B Antigens
DNA
Regulator Genes
HLA Antigens
Endoplasmic Reticulum
Genes
Immune System
Fetus
Gene Expression
Peptides

ASJC Scopus subject areas

  • Genetics

Cite this

Ikeno, M., Suzuki, N., Kamiya, M., Takahashi, Y., Kudo, J., & Okazaki, T. (2012). LINE1 family member is negative regulator of HLA-G expression. Nucleic Acids Research, 40(21), 10742-10752. https://doi.org/10.1093/nar/gks874

LINE1 family member is negative regulator of HLA-G expression. / Ikeno, Masashi; Suzuki, Nobutaka; Kamiya, Megumi; Takahashi, Yuji; Kudo, Jun; Okazaki, Tsuneko.

In: Nucleic Acids Research, Vol. 40, No. 21, 11.2012, p. 10742-10752.

Research output: Contribution to journalArticle

Ikeno, M, Suzuki, N, Kamiya, M, Takahashi, Y, Kudo, J & Okazaki, T 2012, 'LINE1 family member is negative regulator of HLA-G expression', Nucleic Acids Research, vol. 40, no. 21, pp. 10742-10752. https://doi.org/10.1093/nar/gks874
Ikeno M, Suzuki N, Kamiya M, Takahashi Y, Kudo J, Okazaki T. LINE1 family member is negative regulator of HLA-G expression. Nucleic Acids Research. 2012 Nov;40(21):10742-10752. https://doi.org/10.1093/nar/gks874
Ikeno, Masashi ; Suzuki, Nobutaka ; Kamiya, Megumi ; Takahashi, Yuji ; Kudo, Jun ; Okazaki, Tsuneko. / LINE1 family member is negative regulator of HLA-G expression. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 21. pp. 10742-10752.
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