LINE1 family member is negative regulator of HLA-G expression

Masashi Ikeno, Nobutaka Suzuki, Megumi Kamiya, Yuji Takahashi, Jun Kudoh, Tsuneko Okazaki

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Abstract

Class Ia molecules of human leucocyte antigen (HLA-A,-B and-C) are widely expressed and play a central role in the immune system by presenting peptides derived from the lumen of the endoplasmic reticulum. In contrast, class Ib molecules such as HLA-G serve novel functions. The distribution of HLA-G is mostly limited to foetal trophoblastic tissues and some tumour tissues. The mechanism required for the tissue-specific regulation of the HLA-G gene has not been well understood. Here, we investigated the genomic regulation of HLA-G by manipulating one copy of a genomic DNA fragment on a human artificial chromosome. We identified a potential negative regulator of gene expression in a sequence upstream of HLA-G that overlapped with the long interspersed element (LINE1); silencing of HLA-G involved a DNA secondary structure generated in LINE1. The presence of a LINE1 gene silencer may explain the limited expression of HLA-G compared with other class I genes.

Original languageEnglish
Pages (from-to)10742-10752
Number of pages11
JournalNucleic acids research
Volume40
Issue number21
DOIs
Publication statusPublished - 2012 Nov 1

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ASJC Scopus subject areas

  • Genetics

Cite this

Ikeno, M., Suzuki, N., Kamiya, M., Takahashi, Y., Kudoh, J., & Okazaki, T. (2012). LINE1 family member is negative regulator of HLA-G expression. Nucleic acids research, 40(21), 10742-10752. https://doi.org/10.1093/nar/gks874