Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain

Hiroyuki Matsumura, Hidetoshi Hasuwa, Naokazu Inoue, Masahito Ikawa, Masaru Okabe

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We have established a transgenic mouse line in which floxed neomycin resistant cassette was inserted between the CAG promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number2
DOIs
Publication statusPublished - 2004 Aug 20
Externally publishedYes

Keywords

  • Animal model for human disease
  • Cell lineage specific disruption
  • Cre
  • Diphtheria toxin
  • EGFP
  • LoxP
  • Transgenic mouse

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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