Lipoproteins regulate C-type natriuretic peptide secretion from cultured vascular endothelial cells

S. Sugiyama, K. Kugiyama, T. Matsumura, S. I. Suga, Hiroshi Itoh, K. Nakao, H. Yasue

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We have shown that oxidized low-density lipoprotein (Ox-LDL) modulates various endothelial cell (EC) functions. C-type natriuretic peptide (CNP), the third member of the natriuretic peptide family to be discovered, is secreted from peripheral vascular ECs and regulates body fluid homeostasis, vascular tone, and vascular growth. This study was designed to investigate the effects of lipoproteins on CNP secretion from cultured ECs. Treatment of bovine carotid ECs with Ox-LDL and its extracted lipids resulted in a concentration-dependent suppression of the spontaneous and transforming growth factor-β1-stimulated secretion of CNP. Native LDL, its extracted lipids, and acetylated LDL were inactive. Ox-LDL depleted of its amphiphilic lipids, which was prepared by incubation with defatted albumin, lost its suppressive effect on CNP secretion. 7-Ketocholesterol, one of the amphiphilic lipids in Ox-LDL that is transferable from Ox-LDL to defatted albumin, suppressed CNP secretion by ECs, thus mimicking the effect of Ox- LDL. Coincubation with high-density lipoprotein (HDL), which alone had no effect on CNP release, significantly prevented Ox-LDL-induced inhibition of CNP secretion by ECs. Analysis by thin-layer chromatography demonstrated that oxysterols, including 7-ketocholesterol, in Ox-LDL were transferred from Ox- LDL to HDL during coincubation of these two lipoproteins. These results indicate that Ox-LDL suppresses CNP secretion from ECs by 7-ketocholesterol or other transferable hydrophilic lipids in Ox-LDL, and the suppressive effect of Ox-LDL is reversed by HDL. Lipoproteins thus may regulate CNP secretion from the endothelium of atherosclerotic arteries.

Original languageEnglish
Pages (from-to)1968-1974
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume15
Issue number11
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Lipoproteins
Endothelial Cells
Lipids
HDL Lipoproteins
Blood Vessels
oxidized low density lipoprotein
Albumins
Natriuretic Peptides
Transforming Growth Factors
Body Fluids
Thin Layer Chromatography

Keywords

  • 7-ketocholesterol
  • C-type natriuretic peptide
  • endothelial cells
  • oxidized LDL
  • oxysterols

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Sugiyama, S., Kugiyama, K., Matsumura, T., Suga, S. I., Itoh, H., Nakao, K., & Yasue, H. (1995). Lipoproteins regulate C-type natriuretic peptide secretion from cultured vascular endothelial cells. Arteriosclerosis, Thrombosis, and Vascular Biology, 15(11), 1968-1974.

Lipoproteins regulate C-type natriuretic peptide secretion from cultured vascular endothelial cells. / Sugiyama, S.; Kugiyama, K.; Matsumura, T.; Suga, S. I.; Itoh, Hiroshi; Nakao, K.; Yasue, H.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 15, No. 11, 1995, p. 1968-1974.

Research output: Contribution to journalArticle

Sugiyama, S, Kugiyama, K, Matsumura, T, Suga, SI, Itoh, H, Nakao, K & Yasue, H 1995, 'Lipoproteins regulate C-type natriuretic peptide secretion from cultured vascular endothelial cells', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 15, no. 11, pp. 1968-1974.
Sugiyama, S. ; Kugiyama, K. ; Matsumura, T. ; Suga, S. I. ; Itoh, Hiroshi ; Nakao, K. ; Yasue, H. / Lipoproteins regulate C-type natriuretic peptide secretion from cultured vascular endothelial cells. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1995 ; Vol. 15, No. 11. pp. 1968-1974.
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