Liposomes with temperature-responsive reversible surface properties

Ryo Nemoto, Kei Fujieda, Yuki Hiruta, Mafumi Hishida, Eri Ayano, Yoshie Maitani, Kenichi Nagase, Hideko Kanazawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Liposomes composed of egg phosphatidylcholine and cholesterol were modified with the temperature-responsive polymer poly(N-isopropylacrylamide-co-N, N-dimethylacrylamide) (P(NIPAAm-co-DMAAm)), and exhibited reversible surface properties with temperature. Completely reversible liposome aggregation due to P(NIPAAm-co-DMAAm) hydration/dehydration was demonstrated over four successive cycles of heating and cooling. The P(NIPAAm-co-DMAAm) polymer was hydrated during cooling, which dispersed the liposomes. The rigidity of the liposomal membrane was one of the factors in the reversible aggregation, as was the modification density of the polymer on the liposomes. A low density on relatively rigid liposomes could maintain the polymer property of reversible hydrated layers below critical solution temperature (LCST) boundary. Above the LCST, temperature-responsive polymers could also transport negatively charged liposomes into cells. The reversible behavior of the temperature-responsive polymer-modified liposomes has not been reported previously and could enable new applications for switching deposit forms as alternative drug carriers.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume176
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Surface Properties
Liposomes
surface properties
Surface properties
Polymers
Temperature
polymers
temperature
Agglomeration
cooling
Cooling
eggs
cholesterol
Drug Carriers
rigidity
Cholesterol
dehydration
hydration
Phosphatidylcholines
Dehydration

Keywords

  • Cellular uptake
  • P(NIPAAm-co-DMAAm)-modified liposomes
  • Reversible switching surface property
  • Temperature-responsive liposome

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Liposomes with temperature-responsive reversible surface properties. / Nemoto, Ryo; Fujieda, Kei; Hiruta, Yuki; Hishida, Mafumi; Ayano, Eri; Maitani, Yoshie; Nagase, Kenichi; Kanazawa, Hideko.

In: Colloids and Surfaces B: Biointerfaces, Vol. 176, 01.04.2019, p. 309-316.

Research output: Contribution to journalArticle

Nemoto, Ryo ; Fujieda, Kei ; Hiruta, Yuki ; Hishida, Mafumi ; Ayano, Eri ; Maitani, Yoshie ; Nagase, Kenichi ; Kanazawa, Hideko. / Liposomes with temperature-responsive reversible surface properties. In: Colloids and Surfaces B: Biointerfaces. 2019 ; Vol. 176. pp. 309-316.
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AU - Maitani, Yoshie

AU - Nagase, Kenichi

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