Loading of mechanical pressure activates mitogen-activated protein kinase and early immediate gene in intestinal epithelial cells

Masahiko Hirokawa, Soichiro Miura, Hiroshi Kishikawa, Hideo Yoshida, Hiromasa Nakamizo, Hajime Higuchi, Ruri C. Nakatsumi, Hidekazu Suzuki, Hidetsugu Saito, Hiromasa Ishii

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Intestinal mucosa is continuously exposed to mechanical forces. We examined whether pressure loading activates mitogen-activated protein kinase (MAPK) and expression of early immediate genes in intestinal epithelial cells. Pressure was applied to IEC18 cells by helium gas in a culture flask and pressure-induced cell proliferation was examined. The expression of early immediate genes, MAPK activity, and activation of nuclear factor activator protein-1 (AP-1) were also examined. Pressures significantly promoted cell proliferation with peak effect at 80 mm Hg. Pretreatment with either a protein kinase C inhibitor or tyrosine kinase inhibitors, but not calcium chelating agents significantly inhibited cell proliferation promoted by pressure. Early inductions of c-myc and c-fos proteins, increased activity of MAPK, and activation of AP-1 were observed by pressure loading. Our study showed that intestinal mucosal cell proliferation is promoted by mechanical pressure and various intracellular signaling pathways are involved in the process.

Original languageEnglish
Pages (from-to)1993-2003
Number of pages11
JournalDigestive Diseases and Sciences
Volume46
Issue number9
DOIs
Publication statusPublished - 2001

Keywords

  • Cell proliferation
  • Nuclear factor activator protein-1
  • Protein kinase C
  • Tyrosine kinase

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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    Hirokawa, M., Miura, S., Kishikawa, H., Yoshida, H., Nakamizo, H., Higuchi, H., Nakatsumi, R. C., Suzuki, H., Saito, H., & Ishii, H. (2001). Loading of mechanical pressure activates mitogen-activated protein kinase and early immediate gene in intestinal epithelial cells. Digestive Diseases and Sciences, 46(9), 1993-2003. https://doi.org/10.1023/A:1010607819842