Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Kenichi Nagase, Yuhei Nagumo, Miri Kim, Hee Jung Kim, Hei Won Kyung, Hye Jin Chung, Hidekazu Sekine, Tatsuya Shimizu, Hideko Kanazawa, Teruo Okano, Seung Jin Lee, Masayuki Yamato

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.

Original languageEnglish
JournalMacromolecular Bioscience
DOIs
Publication statusAccepted/In press - 2017

Keywords

  • Cell sheet
  • Electrospun fiber
  • Regenerative medicine
  • Tissue engineering
  • VEGF

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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  • Cite this

    Nagase, K., Nagumo, Y., Kim, M., Kim, H. J., Kyung, H. W., Chung, H. J., Sekine, H., Shimizu, T., Kanazawa, H., Okano, T., Lee, S. J., & Yamato, M. (Accepted/In press). Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets. Macromolecular Bioscience. https://doi.org/10.1002/mabi.201700073