Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets

Kenichi Nagase, Yuhei Nagumo, Miri Kim, Hee Jung Kim, Hei Won Kyung, Hye Jin Chung, Hidekazu Sekine, Tatsuya Shimizu, Hideko Kanazawa, Teruo Okano, Seung Jin Lee, Masayuki Yamato

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes. Thus, local VEGF release near the transplanted cardiomyocytes induces vascularization, which supplies sufficient oxygen and nutrients to prevent necrosis. In contrast, cardiomyocyte sheets without a VEGF-releasing mat do not survive in vivo, probably undergo necrosis, and are reduced in thickness. Hence, these VEGF-releasing mats enable the transplantation of multilayered cardiomyocyte sheets in a single procedure, and should expand the potential of cell sheet transplantation for therapeutic applications.

Original languageEnglish
JournalMacromolecular Bioscience
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Cardiac Myocytes
Vascular Endothelial Growth Factor A
Transplantation
Fibers
Cell Transplantation
Necrosis
Bearings (structural)
Nude Rats
Transplantation (surgical)
Oxygen supply
Engineering technology
Blood vessels
Electrospinning
Tissue Engineering
Intercellular Signaling Peptides and Proteins
Tissue engineering
Nanoparticles
Nutrients
Blood Vessels
Rats

Keywords

  • Cell sheet
  • Electrospun fiber
  • Regenerative medicine
  • Tissue engineering
  • VEGF

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets. / Nagase, Kenichi; Nagumo, Yuhei; Kim, Miri; Kim, Hee Jung; Kyung, Hei Won; Chung, Hye Jin; Sekine, Hidekazu; Shimizu, Tatsuya; Kanazawa, Hideko; Okano, Teruo; Lee, Seung Jin; Yamato, Masayuki.

In: Macromolecular Bioscience, 2017.

Research output: Contribution to journalArticle

Nagase, Kenichi ; Nagumo, Yuhei ; Kim, Miri ; Kim, Hee Jung ; Kyung, Hei Won ; Chung, Hye Jin ; Sekine, Hidekazu ; Shimizu, Tatsuya ; Kanazawa, Hideko ; Okano, Teruo ; Lee, Seung Jin ; Yamato, Masayuki. / Local Release of VEGF Using Fiber Mats Enables Effective Transplantation of Layered Cardiomyocyte Sheets. In: Macromolecular Bioscience. 2017.
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AU - Chung, Hye Jin

AU - Sekine, Hidekazu

AU - Shimizu, Tatsuya

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AU - Okano, Teruo

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