Localization and synthesis of acetylcholine in human leukemic T cell lines

T. Fujii, T. Tsuchiya, S. Yamada, Kazuko Fujimoto, Takeshi Suzuki, T. Kasahara, K. Kawashima

Research output: Contribution to journalArticle

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Abstract

In order to clarify the origin of acetylcholine (ACh) in human blood, we measured the content and synthesis activity of ACh in several human leukemic cell lines. The intracellular ACh content determined by a specific and sensitive radioimmunoassay in the human leukemic T cell lines, HSB-2, MOLT- 3, and CEM, was 79.6, 36.2, and 9.5 pmol/106 cells, respectively. These values were 9-70-fold higher than those of other cell lines, including a helper T cell line, Jurkat. Stimulation of HSB-2 and MOLT-3 by phytohemagglutinin (PHA) increased both the intracellular content and release of ACh into the culture medium, but did not influence the intracellular content and release of ACh in CEM. ACh synthesis activity was found in all the T cell lines tested. Bromoacetylcholine (100 μM), a choline acetyltransferase (CHAT) inhibitor, and bromoacetyl-L-carnitine (100 μM), a carnitine acetyltransferase (CarAT) inhibitor, decreased ACh-synthesizing activity in MOLT-3, and HSB-2 and CEM, by about 50% and 30%, respectively, indicating that both CHAT, and to a lesser extent CarAT, are involved in ACh synthesis in T cells. These results suggest that T lymphocytes have the potential to synthesize and release ACh, which may play a role in regulating T cell-dependent immune responses.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalJournal of Neuroscience Research
Volume44
Issue number1
DOIs
Publication statusPublished - 1996

Fingerprint

Acetylcholine
T-Lymphocytes
Cell Line
Carnitine O-Acetyltransferase
Choline O-Acetyltransferase
Carnitine
Phytohemagglutinins
Helper-Inducer T-Lymphocytes
Radioimmunoassay
Culture Media

Keywords

  • acetylcholine
  • carnitine acetyltransferase
  • choline acetyltransferase
  • human leukemic T cell line
  • T lymphocyte

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization and synthesis of acetylcholine in human leukemic T cell lines. / Fujii, T.; Tsuchiya, T.; Yamada, S.; Fujimoto, Kazuko; Suzuki, Takeshi; Kasahara, T.; Kawashima, K.

In: Journal of Neuroscience Research, Vol. 44, No. 1, 1996, p. 66-72.

Research output: Contribution to journalArticle

Fujii, T. ; Tsuchiya, T. ; Yamada, S. ; Fujimoto, Kazuko ; Suzuki, Takeshi ; Kasahara, T. ; Kawashima, K. / Localization and synthesis of acetylcholine in human leukemic T cell lines. In: Journal of Neuroscience Research. 1996 ; Vol. 44, No. 1. pp. 66-72.
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