Localization of α-tocopherol transfer protein in rat brain

Akihiro Hosomi, Kaoru Goto, Hisatake Kondo, Takeshi Iwatsubo, Takanori Yokota, Masako Ogawa, Makoto Arita, Junken Aoki, Hiroyuki Arai, Keizo Inoue

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Vitamin E (α-tocopherol) is a fat-soluble antioxidant that is transported by plasma lipoproteins in the body. α-Tocopherol transfer protein (α-TTP), which was identified as a product of the causative gene for familial isolated vitamin E (FIVE) deficiency, is a cytosolic liver protein which plays an important role in the efficient circulation of plasma vitamin E in the body. In the present study, we detected the message for α-TTP at low levels in some rat tissues including brain, spleen, lung and kidney. In the brain, the α-TTP transcript was detected predominantly in the cerebellar cortex, as revealed by in situ hybridization histochemistry. In the cerebellar cortex, clusters of the hybridization signal were aligned with the Purkinje cell layer, although the signal was not detected in the Purkinje cells, but in the small cells around the Purkinje cells. This distribution pattern strongly suggests that the message for α-TTP is expressed in the Bergmann glial cells. Combined with the previous observation that there was severe Purkinje cell loss in patients with FIVE deficiency, the present data suggest that vitamin E is supplied to the Purkinje cells from the surrounding Bergmann glial cells with the help of α-TTP.

Original languageEnglish
Pages (from-to)159-162
Number of pages4
JournalNeuroscience Letters
Volume256
Issue number3
DOIs
Publication statusPublished - 1998 Nov 13
Externally publishedYes

Fingerprint

Tocopherols
Purkinje Cells
Vitamin E
Brain
Cerebellar Cortex
Proteins
Neuroglia
Lipoproteins
In Situ Hybridization
Spleen
Antioxidants
Fats
Kidney
Lung
thymidine 5'-triphosphate
Liver
Genes

Keywords

  • α-Tocopherol transfer protein
  • Familial isolated vitamin E deficiency
  • Vitamin E

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hosomi, A., Goto, K., Kondo, H., Iwatsubo, T., Yokota, T., Ogawa, M., ... Inoue, K. (1998). Localization of α-tocopherol transfer protein in rat brain. Neuroscience Letters, 256(3), 159-162. https://doi.org/10.1016/S0304-3940(98)00785-X

Localization of α-tocopherol transfer protein in rat brain. / Hosomi, Akihiro; Goto, Kaoru; Kondo, Hisatake; Iwatsubo, Takeshi; Yokota, Takanori; Ogawa, Masako; Arita, Makoto; Aoki, Junken; Arai, Hiroyuki; Inoue, Keizo.

In: Neuroscience Letters, Vol. 256, No. 3, 13.11.1998, p. 159-162.

Research output: Contribution to journalArticle

Hosomi, A, Goto, K, Kondo, H, Iwatsubo, T, Yokota, T, Ogawa, M, Arita, M, Aoki, J, Arai, H & Inoue, K 1998, 'Localization of α-tocopherol transfer protein in rat brain', Neuroscience Letters, vol. 256, no. 3, pp. 159-162. https://doi.org/10.1016/S0304-3940(98)00785-X
Hosomi A, Goto K, Kondo H, Iwatsubo T, Yokota T, Ogawa M et al. Localization of α-tocopherol transfer protein in rat brain. Neuroscience Letters. 1998 Nov 13;256(3):159-162. https://doi.org/10.1016/S0304-3940(98)00785-X
Hosomi, Akihiro ; Goto, Kaoru ; Kondo, Hisatake ; Iwatsubo, Takeshi ; Yokota, Takanori ; Ogawa, Masako ; Arita, Makoto ; Aoki, Junken ; Arai, Hiroyuki ; Inoue, Keizo. / Localization of α-tocopherol transfer protein in rat brain. In: Neuroscience Letters. 1998 ; Vol. 256, No. 3. pp. 159-162.
@article{f9ba87e9ffc74f318d2244a53822f463,
title = "Localization of α-tocopherol transfer protein in rat brain",
abstract = "Vitamin E (α-tocopherol) is a fat-soluble antioxidant that is transported by plasma lipoproteins in the body. α-Tocopherol transfer protein (α-TTP), which was identified as a product of the causative gene for familial isolated vitamin E (FIVE) deficiency, is a cytosolic liver protein which plays an important role in the efficient circulation of plasma vitamin E in the body. In the present study, we detected the message for α-TTP at low levels in some rat tissues including brain, spleen, lung and kidney. In the brain, the α-TTP transcript was detected predominantly in the cerebellar cortex, as revealed by in situ hybridization histochemistry. In the cerebellar cortex, clusters of the hybridization signal were aligned with the Purkinje cell layer, although the signal was not detected in the Purkinje cells, but in the small cells around the Purkinje cells. This distribution pattern strongly suggests that the message for α-TTP is expressed in the Bergmann glial cells. Combined with the previous observation that there was severe Purkinje cell loss in patients with FIVE deficiency, the present data suggest that vitamin E is supplied to the Purkinje cells from the surrounding Bergmann glial cells with the help of α-TTP.",
keywords = "α-Tocopherol transfer protein, Familial isolated vitamin E deficiency, Vitamin E",
author = "Akihiro Hosomi and Kaoru Goto and Hisatake Kondo and Takeshi Iwatsubo and Takanori Yokota and Masako Ogawa and Makoto Arita and Junken Aoki and Hiroyuki Arai and Keizo Inoue",
year = "1998",
month = "11",
day = "13",
doi = "10.1016/S0304-3940(98)00785-X",
language = "English",
volume = "256",
pages = "159--162",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Localization of α-tocopherol transfer protein in rat brain

AU - Hosomi, Akihiro

AU - Goto, Kaoru

AU - Kondo, Hisatake

AU - Iwatsubo, Takeshi

AU - Yokota, Takanori

AU - Ogawa, Masako

AU - Arita, Makoto

AU - Aoki, Junken

AU - Arai, Hiroyuki

AU - Inoue, Keizo

PY - 1998/11/13

Y1 - 1998/11/13

N2 - Vitamin E (α-tocopherol) is a fat-soluble antioxidant that is transported by plasma lipoproteins in the body. α-Tocopherol transfer protein (α-TTP), which was identified as a product of the causative gene for familial isolated vitamin E (FIVE) deficiency, is a cytosolic liver protein which plays an important role in the efficient circulation of plasma vitamin E in the body. In the present study, we detected the message for α-TTP at low levels in some rat tissues including brain, spleen, lung and kidney. In the brain, the α-TTP transcript was detected predominantly in the cerebellar cortex, as revealed by in situ hybridization histochemistry. In the cerebellar cortex, clusters of the hybridization signal were aligned with the Purkinje cell layer, although the signal was not detected in the Purkinje cells, but in the small cells around the Purkinje cells. This distribution pattern strongly suggests that the message for α-TTP is expressed in the Bergmann glial cells. Combined with the previous observation that there was severe Purkinje cell loss in patients with FIVE deficiency, the present data suggest that vitamin E is supplied to the Purkinje cells from the surrounding Bergmann glial cells with the help of α-TTP.

AB - Vitamin E (α-tocopherol) is a fat-soluble antioxidant that is transported by plasma lipoproteins in the body. α-Tocopherol transfer protein (α-TTP), which was identified as a product of the causative gene for familial isolated vitamin E (FIVE) deficiency, is a cytosolic liver protein which plays an important role in the efficient circulation of plasma vitamin E in the body. In the present study, we detected the message for α-TTP at low levels in some rat tissues including brain, spleen, lung and kidney. In the brain, the α-TTP transcript was detected predominantly in the cerebellar cortex, as revealed by in situ hybridization histochemistry. In the cerebellar cortex, clusters of the hybridization signal were aligned with the Purkinje cell layer, although the signal was not detected in the Purkinje cells, but in the small cells around the Purkinje cells. This distribution pattern strongly suggests that the message for α-TTP is expressed in the Bergmann glial cells. Combined with the previous observation that there was severe Purkinje cell loss in patients with FIVE deficiency, the present data suggest that vitamin E is supplied to the Purkinje cells from the surrounding Bergmann glial cells with the help of α-TTP.

KW - α-Tocopherol transfer protein

KW - Familial isolated vitamin E deficiency

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=0032515089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032515089&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(98)00785-X

DO - 10.1016/S0304-3940(98)00785-X

M3 - Article

C2 - 9855364

AN - SCOPUS:0032515089

VL - 256

SP - 159

EP - 162

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -