Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes

Masanori Taira, Jun Kudo, Shinsei Minoshima, Taizo Iizasa, Hideaki Shimada, Yoshiko Shimizu, Masamiti Tatibana, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

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Abstract

Complementary DNA clones for phosphoribosylpyrophosphate synthetase subunits I and II (PRS I and PRS II) were used to determine the chromosomal localization of the corresponding human genes. Southern blot analysis of genomic DNAs isolated from human placenta and a panel of humanmouse somatic cell hybrids revealed that the rat PRS I cDNA probe detected at least five human specific DNA segments (23, 20, 14.5, 6.7, and 4.3 kb) in BamHI digests. The 23-, 14.5-, and 6.7-kb DNA segments were detected only if the hybrids contained human chromosome X or translocation chromosome 7p+ (7qter>7p22::Xq21>Xqter), indicating the location of these segments to Xq21-qter (PRPS1). The 20- and 4.3-kb DNA segments did not cosegregate with the other three segments, and spot blot hybridization analysis using flow-sorted human chromosomes indicated that these are the PRPS1-related genes (PRPS1L1 and PRPS1L2) and could be assigned to chromosomes 7 and 9, respectively. The human-specific PRS II cDNA probe revealed a BamHI DNA segment (17 kb), which segregated condordantly with the X chromosome but not with the PRPS1 gene. We surmise that the gene for PRS II (PRPS2) is located at a different region of the X chromosome, namely Xpter-q21.

Original languageEnglish
Pages (from-to)29-37
Number of pages9
JournalSomatic Cell and Molecular Genetics
Volume15
Issue number1
DOIs
Publication statusPublished - 1989 Jan

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X Chromosome
Ligases
DNA
Complementary DNA
Genes
Chromosomes, Human, X
Chromosomes, Human, Pair 9
Chromosomes, Human, Pair 7
Hybrid Cells
Human Chromosomes
Southern Blotting
Placenta
Clone Cells
Chromosomes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes. / Taira, Masanori; Kudo, Jun; Minoshima, Shinsei; Iizasa, Taizo; Shimada, Hideaki; Shimizu, Yoshiko; Tatibana, Masamiti; Shimizu, Nobuyoshi.

In: Somatic Cell and Molecular Genetics, Vol. 15, No. 1, 01.1989, p. 29-37.

Research output: Contribution to journalArticle

Taira, Masanori ; Kudo, Jun ; Minoshima, Shinsei ; Iizasa, Taizo ; Shimada, Hideaki ; Shimizu, Yoshiko ; Tatibana, Masamiti ; Shimizu, Nobuyoshi. / Localization of human phosphoribosylpyrophosphate synthetase subunit I and II genes (PRPS1 and PRPS2) to different regions of the X chromosome and assignment of two PRPS1-related genes to autosomes. In: Somatic Cell and Molecular Genetics. 1989 ; Vol. 15, No. 1. pp. 29-37.
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abstract = "Complementary DNA clones for phosphoribosylpyrophosphate synthetase subunits I and II (PRS I and PRS II) were used to determine the chromosomal localization of the corresponding human genes. Southern blot analysis of genomic DNAs isolated from human placenta and a panel of humanmouse somatic cell hybrids revealed that the rat PRS I cDNA probe detected at least five human specific DNA segments (23, 20, 14.5, 6.7, and 4.3 kb) in BamHI digests. The 23-, 14.5-, and 6.7-kb DNA segments were detected only if the hybrids contained human chromosome X or translocation chromosome 7p+ (7qter>7p22::Xq21>Xqter), indicating the location of these segments to Xq21-qter (PRPS1). The 20- and 4.3-kb DNA segments did not cosegregate with the other three segments, and spot blot hybridization analysis using flow-sorted human chromosomes indicated that these are the PRPS1-related genes (PRPS1L1 and PRPS1L2) and could be assigned to chromosomes 7 and 9, respectively. The human-specific PRS II cDNA probe revealed a BamHI DNA segment (17 kb), which segregated condordantly with the X chromosome but not with the PRPS1 gene. We surmise that the gene for PRS II (PRPS2) is located at a different region of the X chromosome, namely Xpter-q21.",
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