Localization of water channels in the skin of two species of desert toads, anaxyrus (Bufo) punctatus and incilius (Bufo) alvarius

Yuki Shibata, Hiro Aki Takeuchi, Takahiro Hasegawa, Masakazu Suzuki, Shigeyasu Tanaka, Stanley D. Hillyard, Takatoshi Nagai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Anuran amphibians obtain water by osmosis across their ventral skin. A specialized region in the pelvic skin of semiterrestrial species, termed the seat patch, contains aquaporins (AQPs) that become inserted into the apical plasma membrane of the epidermis following stimulation by arginine vasotocin (AVT) to facilitate rehydration. Two AVT-stimulated AQPs, AQP-h2 and AQP-h3, have been identified in the epidermis of seat patch skin of the Japanese tree frog, Hyla japonica, and show a high degree of homology with those of bufonid species. We used antibodies raised against AQP-h2 and AQP-h3 to characterize the expression of homologous AQPs in the skin of two species of toads that inhabit arid desert regions of southwestern North America. Western blot analysis of proteins gave positive results for AQP-h2-like proteins in the pelvic skin and also the urinary bladder of Anaxyrus (Bufo) punctatus while AQP-h3-like proteins were found in extracts from the pelvic skin and the more anterior ventral skin, but not the urinary bladder. Immunohistochemical observations showed both AQP-h2-and AQP-h3-like proteins were present in the apical membrane of skin from the pelvic skin of hydrated and dehydrated A. punctatus. Further stimulation by AVT or isoproterenol treatment of living toads was not evident. In contrast, skin from hydrated Incilius (Bufo) alvarius showed very weak labeling of AQP-h2-and AQP-h3-like proteins and labeling turned intense following stimulation by AVT. These results are similar to those of tree frogs and toads that occupy mesic habitats and suggest this pattern of AQP expression is the result of phylogenetic factors shared by hylid and bufonid anurans.

Original languageEnglish
Pages (from-to)664-670
Number of pages7
JournalZoological Science
Volume28
Issue number9
DOIs
Publication statusPublished - 2011 Sep

Fingerprint

Bufo
aquaporins
toads
skin (animal)
deserts
arginine vasotocin
water
seats
epidermis (animal)
Hylidae
Anaxyrus
Incilius
bladder
proteins
osmosis
Hyla
rehydration
amphibians

Keywords

  • Anaxyrus punctatus
  • aquaporin
  • arginine vasotocin
  • dehydration tolerance
  • desert toads
  • immunohistochemistry
  • Incilius alvarius
  • ventral pelvic skin

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Shibata, Y., Takeuchi, H. A., Hasegawa, T., Suzuki, M., Tanaka, S., Hillyard, S. D., & Nagai, T. (2011). Localization of water channels in the skin of two species of desert toads, anaxyrus (Bufo) punctatus and incilius (Bufo) alvarius. Zoological Science, 28(9), 664-670. https://doi.org/10.2108/zsj.28.664

Localization of water channels in the skin of two species of desert toads, anaxyrus (Bufo) punctatus and incilius (Bufo) alvarius. / Shibata, Yuki; Takeuchi, Hiro Aki; Hasegawa, Takahiro; Suzuki, Masakazu; Tanaka, Shigeyasu; Hillyard, Stanley D.; Nagai, Takatoshi.

In: Zoological Science, Vol. 28, No. 9, 09.2011, p. 664-670.

Research output: Contribution to journalArticle

Shibata, Y, Takeuchi, HA, Hasegawa, T, Suzuki, M, Tanaka, S, Hillyard, SD & Nagai, T 2011, 'Localization of water channels in the skin of two species of desert toads, anaxyrus (Bufo) punctatus and incilius (Bufo) alvarius', Zoological Science, vol. 28, no. 9, pp. 664-670. https://doi.org/10.2108/zsj.28.664
Shibata, Yuki ; Takeuchi, Hiro Aki ; Hasegawa, Takahiro ; Suzuki, Masakazu ; Tanaka, Shigeyasu ; Hillyard, Stanley D. ; Nagai, Takatoshi. / Localization of water channels in the skin of two species of desert toads, anaxyrus (Bufo) punctatus and incilius (Bufo) alvarius. In: Zoological Science. 2011 ; Vol. 28, No. 9. pp. 664-670.
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abstract = "Anuran amphibians obtain water by osmosis across their ventral skin. A specialized region in the pelvic skin of semiterrestrial species, termed the seat patch, contains aquaporins (AQPs) that become inserted into the apical plasma membrane of the epidermis following stimulation by arginine vasotocin (AVT) to facilitate rehydration. Two AVT-stimulated AQPs, AQP-h2 and AQP-h3, have been identified in the epidermis of seat patch skin of the Japanese tree frog, Hyla japonica, and show a high degree of homology with those of bufonid species. We used antibodies raised against AQP-h2 and AQP-h3 to characterize the expression of homologous AQPs in the skin of two species of toads that inhabit arid desert regions of southwestern North America. Western blot analysis of proteins gave positive results for AQP-h2-like proteins in the pelvic skin and also the urinary bladder of Anaxyrus (Bufo) punctatus while AQP-h3-like proteins were found in extracts from the pelvic skin and the more anterior ventral skin, but not the urinary bladder. Immunohistochemical observations showed both AQP-h2-and AQP-h3-like proteins were present in the apical membrane of skin from the pelvic skin of hydrated and dehydrated A. punctatus. Further stimulation by AVT or isoproterenol treatment of living toads was not evident. In contrast, skin from hydrated Incilius (Bufo) alvarius showed very weak labeling of AQP-h2-and AQP-h3-like proteins and labeling turned intense following stimulation by AVT. These results are similar to those of tree frogs and toads that occupy mesic habitats and suggest this pattern of AQP expression is the result of phylogenetic factors shared by hylid and bufonid anurans.",
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