Logic gate operation by DNA translocation through biological nanopores

Hiroki Yasuga, Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Logical operations using biological molecules, such as DNA computing or programmable diagnosis using DNA, have recently received attention. Challenges remain with respect to the development of such systems, including label-free output detection and the rapidity of operation. Here, we propose integration of biological nanopores with DNA molecules for development of a logical operating system. We configured outputs "1" and "0" as singlestranded DNA (ssDNA) that is or is not translocated through a nanopore; unlabeled DNA was detected electrically. A negative-AND (NAND) operation was successfully conducted within approximately 10 min, which is rapid compared with previous studies using unlabeled DNA. In addition, this operation was executed in a four-droplet network. DNA molecules and associated information were transferred among droplets via biological nanopores. This system would facilitate linking of molecules and electronic interfaces. Thus, it could be applied to molecular robotics, genetic engineering, and even medical diagnosis and treatment.

Original languageEnglish
Article numbere0149667
JournalPLoS One
Volume11
Issue number2
DOIs
Publication statusPublished - 2016 Feb 1

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Nanopores
Logic gates
DNA
Molecules
droplets
Genetic engineering
Genetic Engineering
Robotics
nanopores
genetic engineering
electronics
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Molecular Biology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Yasuga, H., Kawano, R., Takinoue, M., Tsuji, Y., Osaki, T., Kamiya, K., ... Takeuchi, S. (2016). Logic gate operation by DNA translocation through biological nanopores. PLoS One, 11(2), [e0149667]. https://doi.org/10.1371/journal.pone.0149667

Logic gate operation by DNA translocation through biological nanopores. / Yasuga, Hiroki; Kawano, Ryuji; Takinoue, Masahiro; Tsuji, Yutaro; Osaki, Toshihisa; Kamiya, Koki; Miki, Norihisa; Takeuchi, Shoji.

In: PLoS One, Vol. 11, No. 2, e0149667, 01.02.2016.

Research output: Contribution to journalArticle

Yasuga, H, Kawano, R, Takinoue, M, Tsuji, Y, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2016, 'Logic gate operation by DNA translocation through biological nanopores', PLoS One, vol. 11, no. 2, e0149667. https://doi.org/10.1371/journal.pone.0149667
Yasuga H, Kawano R, Takinoue M, Tsuji Y, Osaki T, Kamiya K et al. Logic gate operation by DNA translocation through biological nanopores. PLoS One. 2016 Feb 1;11(2). e0149667. https://doi.org/10.1371/journal.pone.0149667
Yasuga, Hiroki ; Kawano, Ryuji ; Takinoue, Masahiro ; Tsuji, Yutaro ; Osaki, Toshihisa ; Kamiya, Koki ; Miki, Norihisa ; Takeuchi, Shoji. / Logic gate operation by DNA translocation through biological nanopores. In: PLoS One. 2016 ; Vol. 11, No. 2.
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