Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore

Hiroki Yasuga, Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while the output was obtained by electrical signals across the nanopores, which recognizes whether ssDNA passed through the nanopores or not. NAND gate was successfully demonstrated by exploiting the mechanism. The proposed approach herein is significantly different from the conventional computation using DNA in the respect that electrical signals are directly obtained as the output, which drastically facilities the microfluidic system to connect to electrical systems for fast and accurate computing. In addition, it is not required to use fluorescence, enzyme or PCR in order to obtain outputs. We believe that this method leads to a rapid computing system using biomolecules.

Original languageEnglish
Title of host publicationProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Pages1005-1006
Number of pages2
DOIs
Publication statusPublished - 2013
EventIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China
Duration: 2013 Jan 202013 Jan 24

Other

OtherIEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
CountryTaiwan, Province of China
CityTaipei
Period13/1/2013/1/24

Fingerprint

Nanopores
Logic gates
Cell membranes
logic
output
Single-Stranded DNA
deoxyribonucleic acid
Microfluidics
DNA
Lipid bilayers
lipids
enzymes
Biomolecules
Membrane Lipids
membranes
fluorescence
Enzymes
Fluorescence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Yasuga, H., Kawano, R., Takinoue, M., Tsuji, Y., Osaki, T., Kamiya, K., ... Takeuchi, S. (2013). Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) (pp. 1005-1006). [6474417] https://doi.org/10.1109/MEMSYS.2013.6474417

Logic gate using artificial cell-membrane : NAND operation by transmembrane DNA via a biological nanopore. / Yasuga, Hiroki; Kawano, Ryuji; Takinoue, Masahiro; Tsuji, Yutaro; Osaki, Toshihisa; Kamiya, Koki; Miki, Norihisa; Takeuchi, Shoji.

Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 1005-1006 6474417.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yasuga, H, Kawano, R, Takinoue, M, Tsuji, Y, Osaki, T, Kamiya, K, Miki, N & Takeuchi, S 2013, Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore. in Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)., 6474417, pp. 1005-1006, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474417
Yasuga H, Kawano R, Takinoue M, Tsuji Y, Osaki T, Kamiya K et al. Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore. In Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. p. 1005-1006. 6474417 https://doi.org/10.1109/MEMSYS.2013.6474417
Yasuga, Hiroki ; Kawano, Ryuji ; Takinoue, Masahiro ; Tsuji, Yutaro ; Osaki, Toshihisa ; Kamiya, Koki ; Miki, Norihisa ; Takeuchi, Shoji. / Logic gate using artificial cell-membrane : NAND operation by transmembrane DNA via a biological nanopore. Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS). 2013. pp. 1005-1006
@inproceedings{027a2003c7344e38aefaa6f19d403b62,
title = "Logic gate using artificial cell-membrane: NAND operation by transmembrane DNA via a biological nanopore",
abstract = "This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while the output was obtained by electrical signals across the nanopores, which recognizes whether ssDNA passed through the nanopores or not. NAND gate was successfully demonstrated by exploiting the mechanism. The proposed approach herein is significantly different from the conventional computation using DNA in the respect that electrical signals are directly obtained as the output, which drastically facilities the microfluidic system to connect to electrical systems for fast and accurate computing. In addition, it is not required to use fluorescence, enzyme or PCR in order to obtain outputs. We believe that this method leads to a rapid computing system using biomolecules.",
author = "Hiroki Yasuga and Ryuji Kawano and Masahiro Takinoue and Yutaro Tsuji and Toshihisa Osaki and Koki Kamiya and Norihisa Miki and Shoji Takeuchi",
year = "2013",
doi = "10.1109/MEMSYS.2013.6474417",
language = "English",
isbn = "9781467356558",
pages = "1005--1006",
booktitle = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

}

TY - GEN

T1 - Logic gate using artificial cell-membrane

T2 - NAND operation by transmembrane DNA via a biological nanopore

AU - Yasuga, Hiroki

AU - Kawano, Ryuji

AU - Takinoue, Masahiro

AU - Tsuji, Yutaro

AU - Osaki, Toshihisa

AU - Kamiya, Koki

AU - Miki, Norihisa

AU - Takeuchi, Shoji

PY - 2013

Y1 - 2013

N2 - This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while the output was obtained by electrical signals across the nanopores, which recognizes whether ssDNA passed through the nanopores or not. NAND gate was successfully demonstrated by exploiting the mechanism. The proposed approach herein is significantly different from the conventional computation using DNA in the respect that electrical signals are directly obtained as the output, which drastically facilities the microfluidic system to connect to electrical systems for fast and accurate computing. In addition, it is not required to use fluorescence, enzyme or PCR in order to obtain outputs. We believe that this method leads to a rapid computing system using biomolecules.

AB - This paper describes microfluidic logic gates which use DNA and biological nanopores. Single-stranded DNA (ssDNA) can pass through αHL, a biological nanopore, incorporated in bilayer lipid membranes (BLMs), whereas double-stranded DNA (dsDNA) cannot. In this study, these passing and non-passing phenomena were applied as the binary system and logic gates. Two types of ssDNA were used as inputs, while the output was obtained by electrical signals across the nanopores, which recognizes whether ssDNA passed through the nanopores or not. NAND gate was successfully demonstrated by exploiting the mechanism. The proposed approach herein is significantly different from the conventional computation using DNA in the respect that electrical signals are directly obtained as the output, which drastically facilities the microfluidic system to connect to electrical systems for fast and accurate computing. In addition, it is not required to use fluorescence, enzyme or PCR in order to obtain outputs. We believe that this method leads to a rapid computing system using biomolecules.

UR - http://www.scopus.com/inward/record.url?scp=84875444577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875444577&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2013.6474417

DO - 10.1109/MEMSYS.2013.6474417

M3 - Conference contribution

AN - SCOPUS:84875444577

SN - 9781467356558

SP - 1005

EP - 1006

BT - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

ER -