Long cellular repeats flanking a defective HTLV-I provirus: Implication for site-targeted integration

Satoshi Kubota, Rika Furuta, Masatoshi Maki, Haruhiko Siomi, Masakazu Hatanaka

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4 Citations (Scopus)

Abstract

Retroviruses generally integrate as proviruses which are flanked by long-terminal repeats (LTRs) on both 5′ and 3′ ends. Since these LTRs are required for the efficient integration mediated by the viral integrase, it is believed that defective proviruses with a single LTR are normally formed by deletion after integration. However, we found no deletion of cellular sequences around the integration site of such a defective HTLV-1. Rather, we identified 99 bp-long direct repeats adjacent to both ends of the defective provirus. The repeated cellular sequences contained a potential poly(A) signal followed by a retroviral primer-binding-site-like sequence. The presence of the direct repeats of cellular sequences can be explained by the integration of the defective virus through homologous recombination between cellular and viral read-through sequences.

Original languageEnglish
Pages (from-to)2873-2877
Number of pages5
JournalOncogene
Volume8
Issue number10
Publication statusPublished - 1993 Oct
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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