Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal

Takeshi Suzuki, Yoshihiro Abe, Daniel S. McGehee, J. Russel Keath, Hiroshi Yajima, Kamal Sharma, James R. Brorson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

For pathophysiological studies, it is advantageous to label specific neuronal populations in living animals. This study aimed to establish a method for stable and long-lasting fluorescent labeling of corticospinal neurons in the living animal. The two fluorescent dyes Fluoro-Red and Fluoro-Green were injected in the cervical spinal cord of anesthetized newborn rats. After a recovery period, treated rats were returned to the mother. After 24 h and 14 days, fixed brain sections revealed wide-spread fluorescence in elongated or pyramidal-shaped cell profiles in a discrete internal cortical layer, consistent with layer V pyramidal cells. Labeled neurons displayed spontaneous synaptic activity using the slice patch clamp method. These results suggest that these dyes are effective tools for pathophysiological and slice patch clamp studies focused on specific neuron groups.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalBrain Research Protocols
Volume13
Issue number3
DOIs
Publication statusPublished - 2004 Aug

Fingerprint

Pyramidal Cells
Neurons
Fluorescent Dyes
Coloring Agents
Fluorescence
Brain
Population
Fluoro-Green
Fluoro-Red
Cervical Cord

Keywords

  • Cortex
  • Corticospinal neuron
  • Fluorescence
  • Fluoro-Green
  • Fluoro-Red
  • Motor systems and sensorimotor integration
  • Rat
  • Retrograde labeling
  • Slice patch clump

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal. / Suzuki, Takeshi; Abe, Yoshihiro; McGehee, Daniel S.; Keath, J. Russel; Yajima, Hiroshi; Sharma, Kamal; Brorson, James R.

In: Brain Research Protocols, Vol. 13, No. 3, 08.2004, p. 183-188.

Research output: Contribution to journalArticle

Suzuki, Takeshi ; Abe, Yoshihiro ; McGehee, Daniel S. ; Keath, J. Russel ; Yajima, Hiroshi ; Sharma, Kamal ; Brorson, James R. / Long-lived retrograde fluorescent labeling of corticospinal neurons in the living animal. In: Brain Research Protocols. 2004 ; Vol. 13, No. 3. pp. 183-188.
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