Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

Asuka Nishino; Yosuke Tajima; Hiroyuki Takuwa; Kazuto Masamoto; Junko Taniguchi; Hidekatsu Wakizaka; Daisuke Kokuryo; Takuya Urushihata; Ichio Aoki; Iwao Kanno; Yutaka Tomita; Norihiro Suzuki; Yoko Ikoma; Hiroshi Ito

doi:10.1038/srep25072

Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

Asuka Nishino, Yosuke Tajima, Hiroyuki Takuwa, Kazuto Masamoto, Junko Taniguchi, Hidekatsu Wakizaka, Daisuke Kokuryo, Takuya Urushihata, Ichio Aoki, Iwao Kanno, Yutaka Tomita, Norihiro Suzuki, Yoko Ikoma, Hiroshi Ito

Department of Internal Medicine

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [11 C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO.

Original language	English
Article number	25072
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep25072
Publication status	Published - 2016 Apr 27

ASJC Scopus subject areas

General

Access to Document

10.1038/srep25072

Cite this

@article{e980c33a342d444697e966c906a8ebc9,

title = "Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model",

abstract = "We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [11 C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO.",

author = "Asuka Nishino and Yosuke Tajima and Hiroyuki Takuwa and Kazuto Masamoto and Junko Taniguchi and Hidekatsu Wakizaka and Daisuke Kokuryo and Takuya Urushihata and Ichio Aoki and Iwao Kanno and Yutaka Tomita and Norihiro Suzuki and Yoko Ikoma and Hiroshi Ito",

year = "2016",

month = apr,

day = "27",

doi = "10.1038/srep25072",

language = "English",

volume = "6",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

AU - Nishino, Asuka

AU - Tajima, Yosuke

AU - Takuwa, Hiroyuki

AU - Masamoto, Kazuto

AU - Taniguchi, Junko

AU - Wakizaka, Hidekatsu

AU - Kokuryo, Daisuke

AU - Urushihata, Takuya

AU - Aoki, Ichio

AU - Kanno, Iwao

AU - Tomita, Yutaka

AU - Suzuki, Norihiro

AU - Ikoma, Yoko

AU - Ito, Hiroshi

PY - 2016/4/27

Y1 - 2016/4/27

N2 - We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [11 C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO.

AB - We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [11 C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO.

UR - http://www.scopus.com/inward/record.url?scp=84964887759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964887759&partnerID=8YFLogxK

U2 - 10.1038/srep25072

DO - 10.1038/srep25072

M3 - Article

C2 - 27116932

AN - SCOPUS:84964887759

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 25072

ER -

Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this