Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model

Asuka Nishino, Yosuke Tajima, Hiroyuki Takuwa, Kazuto Masamoto, Junko Taniguchi, Hidekatsu Wakizaka, Daisuke Kokuryo, Takuya Urushihata, Ichio Aoki, Iwao Kanno, Yutaka Tomita, Norihiro Suzuki, Yoko Ikoma, Hiroshi Ito

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Abstract

We investigated the chronic effects of cerebral hypoperfusion on neuronal density and functional hyperemia using our misery perfusion mouse model under unilateral common carotid artery occlusion (UCCAO). Neuronal density evaluated 28 days after UCCAO using [11 C]flumazenil-PET and histology indicated no neurologic deficit in the hippocampus and neocortex. CBF response to sensory stimulation was assessed using laser-Doppler flowmetry. Percentage changes in CBF response of the ipsilateral hemisphere to UCCAO were 18.4 ± 3.0%, 6.9 ± 2.8%, 6.8 ± 2.3% and 4.9 ± 2.4% before, and 7, 14 and 28 days after UCCAO, respectively. Statistical significance was found at 7, 14 and 28 days after UCCAO (P < 0.01). Contrary to our previous finding (Tajima et al. 2014) showing recovered CBF response to hypercapnia on 28 days after UCCAO using the same model, functional hyperemia was sustained and became worse 28 days after UCCAO.

Original languageEnglish
Article number25072
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Apr 27

ASJC Scopus subject areas

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    Nishino, A., Tajima, Y., Takuwa, H., Masamoto, K., Taniguchi, J., Wakizaka, H., Kokuryo, D., Urushihata, T., Aoki, I., Kanno, I., Tomita, Y., Suzuki, N., Ikoma, Y., & Ito, H. (2016). Long-term effects of cerebral hypoperfusion on neural density and function using misery perfusion animal model. Scientific reports, 6, [25072]. https://doi.org/10.1038/srep25072