TY - JOUR
T1 - Long-term observation of airway reconstruction using decellularized tracheal allografts in micro-miniature pigs at growing stage
AU - Ohno, Michinobu
AU - Fuchimoto, Yasushi
AU - Higuchi, Masataka
AU - Yamaoka, Tetsuji
AU - Komura, Makoto
AU - Umezawa, Akihiro
AU - Hsu, Huai Che
AU - Enosawa, Shin
AU - Kuroda, Tatsuo
N1 - Funding Information:
We thank Mr. Minoru Ichinose, Dr. Hitoshi Abe, and Ms. Hiroko Komura for their excellent technical assistance and Mr. Jyunpei Yamamoto for assistance with the high-pressure treatment of tissues. This work was supported by JSPS KAKENHI [Grant numbers JP16K20348 and JP16K11359 ], AMED under Grant Number JP19bk0104006h0002 , and a Grant-in-Aid from the National Center for Child Health and Development [Grant number 27-8 ].
Publisher Copyright:
© 2020 The Japanese Society for Regenerative Medicine
PY - 2020/12
Y1 - 2020/12
N2 - Introduction: Decellularized tissue exhibits cell matrix-like properties, along with reduced antigenicity. We explored the potential of decellularized allogeneic trachea to restore the upper respiratory tract, focusing on pediatric application. This study specifically aimed at long-term observation of tissue regeneration using a micro-miniature pig model. Methods: Artificial defects (15 × 15 mm) in the subglottis and trachea of micro-miniature pigs were repaired by transplantation of either allogeneic decellularized or fresh (control) tracheal patches. Pigs were evaluated in situ, by bronchoscopy, every three months, and sacrificed for histological examination at six and twelve months after transplantation. Results: No airway symptom was observed in any pig during the observation period. Bronchoscopy revealed the tracheal lumen to be restored by fresh grafts, showing an irregular surface with remarkable longitudinal compression; these changes were mild after restoration with decellularized grafts. Histologically, while fresh graft patches were denatured and replaced by calcified tissue, decellularized patches remained unchanged throughout the observation period. There were regeneration foci of cartilage adjacent to the grafts, and some foci joined the decellularized graft uniformly, suggesting the induction of tracheal reconstitution. Conclusion: Allogeneic decellularized tracheal tissue could serve as a promising biomaterial for tracheal restoration, especially for pediatric patients at the growing stage.
AB - Introduction: Decellularized tissue exhibits cell matrix-like properties, along with reduced antigenicity. We explored the potential of decellularized allogeneic trachea to restore the upper respiratory tract, focusing on pediatric application. This study specifically aimed at long-term observation of tissue regeneration using a micro-miniature pig model. Methods: Artificial defects (15 × 15 mm) in the subglottis and trachea of micro-miniature pigs were repaired by transplantation of either allogeneic decellularized or fresh (control) tracheal patches. Pigs were evaluated in situ, by bronchoscopy, every three months, and sacrificed for histological examination at six and twelve months after transplantation. Results: No airway symptom was observed in any pig during the observation period. Bronchoscopy revealed the tracheal lumen to be restored by fresh grafts, showing an irregular surface with remarkable longitudinal compression; these changes were mild after restoration with decellularized grafts. Histologically, while fresh graft patches were denatured and replaced by calcified tissue, decellularized patches remained unchanged throughout the observation period. There were regeneration foci of cartilage adjacent to the grafts, and some foci joined the decellularized graft uniformly, suggesting the induction of tracheal reconstitution. Conclusion: Allogeneic decellularized tracheal tissue could serve as a promising biomaterial for tracheal restoration, especially for pediatric patients at the growing stage.
KW - Decellularized tracheal tissue
KW - High hydrostatic pressure
KW - Pig
KW - Regeneration
KW - Tissue-engineering
KW - Tracheal restoration
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U2 - 10.1016/j.reth.2020.04.010
DO - 10.1016/j.reth.2020.04.010
M3 - Article
AN - SCOPUS:85088111037
VL - 15
SP - 64
EP - 69
JO - Regenerative Therapy
JF - Regenerative Therapy
SN - 2352-3204
ER -