Long-term, repeated measurements of mouse cortical microflow at the same region of interest with high spatial resolution

Yutaka Tomita, Elisabeth Pinard, Alexy Tran-Dinh, Istvan Schiszler, Nathalie Kubis, Minoru Tomita, Norihiro Suzuki, Jacques Seylaz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A method for long-term, repeated, semi-quantitative measurements of cerebral microflow at the same region of interest (ROI) with high spatial resolution was developed and applied to mice subjected to focal arterial occlusion. A closed cranial window was chronically implanted over the left parieto-occipital cortex. The anesthetized mouse was placed several times, e.g., weekly, under a dynamic confocal microscope, and Rhodamine B-isothiocyanate- dextran was each time intravenously injected as a bolus, while microflow images were video recorded. Left and right tail veins were sequentially catheterized in a mouse three times at maximum over a 1.5 months' observation period. Smearing of the input function resulting from the use of intravenous injection was shown to be sufficiently small. The distal middle cerebral artery (MCA) was thermocoagulated through the cranial window in six mice, and five sham-operated mice were studied in parallel. Dye injection and video recording were conducted four times in this series, i.e., before and at 10 min, 7 and 30 days after sham operation or MCA occlusion. Pixelar microflow values (1/MTT) in a matrix of approximately 50 × 50 pixels were displayed on a two-dimensional (2-D) map, and the frequency distribution of the flow values was also calculated. No significant changes in microflow values over time were detected in sham-operated mice, while the time course of flow changes in the ischemic penumbral area in operated mice was similar to those reported in the literature. This method provides a powerful tool to investigate long-term changes in mouse cortical microflow under physiological and pathological conditions.

Original languageEnglish
Pages (from-to)59-69
Number of pages11
JournalBrain Research
Volume1372
DOIs
Publication statusPublished - 2011 Feb 4

Keywords

  • 2-D flow map
  • Cerebral blood flow
  • Confocal microscopy
  • Focal cerebral ischemia
  • Mouse
  • Optical imaging

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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  • Cite this

    Tomita, Y., Pinard, E., Tran-Dinh, A., Schiszler, I., Kubis, N., Tomita, M., Suzuki, N., & Seylaz, J. (2011). Long-term, repeated measurements of mouse cortical microflow at the same region of interest with high spatial resolution. Brain Research, 1372, 59-69. https://doi.org/10.1016/j.brainres.2010.11.014