Long-term single cell bioluminescence imaging with C-3 position protected coelenterazine analogues

Yuki Mizui, Masatoshi Eguchi, Masanobu Tanaka, Yuma Ikeda, Hideaki Yoshimura, Takeaki Ozawa, Daniel Citterio, Yuki Hiruta

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Bioluminescence is a powerful imaging modality for monitoring biological phenomena bothin vitroandin vivo. Bioluminescence imagin (BLI) is becoming a seamless imaging technology covering the range from cells to organs of small animals. Long-term imaging at the single cell level would lead to a true understanding of the dynamics of life phenomena. This work presents a long-term single cell bioluminescence imaging technology accomplished with C-3 position protected furimazines (FMZs), a CTZ analogues, which generate intense blue emission when paired with a highly stable engineered luciferase, Nanoluc. Four types of FMZs protected at the C-3 position have been synthesized. The type and steric bulkiness of the protection group strongly contributed to storage stability and the kinetics of the bioluminescence reactions of the analogues in human living cells. In particular, two developed FMZ analogues resulted in significantly longer bioluminescence emission with higher S/N ratio than FMZ at single cell level. Long-term bioluminescence single cell imaging technology with the developed FMZ analogues will lead to seamless imaging in the range from cells to organs of small animals.

Original languageEnglish
Pages (from-to)579-586
Number of pages8
JournalOrganic and Biomolecular Chemistry
Volume19
Issue number3
DOIs
Publication statusPublished - 2021 Jan 21

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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