Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid

K. Nagai, Tomoko Betsuyaku, T. Kondo, Y. Nasuhara, M. Nishimura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Epithelial lining fluid plays a critical role in protecting the lung from oxidative stress, in which the oxidised status may change by ageing, smoking history, and pulmonary emphysema. Methods: Bronchoalveolar lavage (BAL) was performed on 109 young and older subjects with various smoking histories. The protein carbonyls, total and oxidised glutathione were examined in BAL fluid. Results: By Western blot analysis, the major carbonylated protein in the BAL fluid was sized at 68 kDa, corresponding to albumin. The amount of carbonylated albumin per mg total albumin in BAL fluid was four times higher in older current smokers and three times higher in older former smokers than in age matched non-smokers (p<0.0001, p = 0.0003, respectively), but not in young smokers. Total glutathione in BAL fluid was significantly increased both in young (p = 0.006) and older current smokers (p = 0.0003) compared with age matched non-smokers. In contrast, the ratio of oxidised to total glutathione was significantly raised (72%) only in older current smokers compared with the other groups. There was no significant difference in these parameters between older smokers with and without mild emphysema. Conclusions: Oxidised glutathione associated with excessive protein carbonylation accumulates in the lung of older smokers with long term smoking histories even in the absence of lung diseases, but they are not significantly enhanced in smokers with mild emphysema.

Original languageEnglish
Pages (from-to)496-502
Number of pages7
JournalThorax
Volume61
Issue number6
DOIs
Publication statusPublished - 2006 Jun
Externally publishedYes

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Bronchoalveolar Lavage Fluid
Oxidative Stress
Smoking
Albumins
Glutathione Disulfide
Emphysema
Glutathione
Protein Carbonylation
Lung
Pulmonary Emphysema
Bronchoalveolar Lavage
Lung Diseases
Proteins
Western Blotting
History

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Nagai, K., Betsuyaku, T., Kondo, T., Nasuhara, Y., & Nishimura, M. (2006). Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid. Thorax, 61(6), 496-502. https://doi.org/10.1136/thx.2005.049148

Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid. / Nagai, K.; Betsuyaku, Tomoko; Kondo, T.; Nasuhara, Y.; Nishimura, M.

In: Thorax, Vol. 61, No. 6, 06.2006, p. 496-502.

Research output: Contribution to journalArticle

Nagai, K, Betsuyaku, T, Kondo, T, Nasuhara, Y & Nishimura, M 2006, 'Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid', Thorax, vol. 61, no. 6, pp. 496-502. https://doi.org/10.1136/thx.2005.049148
Nagai, K. ; Betsuyaku, Tomoko ; Kondo, T. ; Nasuhara, Y. ; Nishimura, M. / Long term smoking with age builds up excessive oxidative stress in bronchoalveolar lavage fluid. In: Thorax. 2006 ; Vol. 61, No. 6. pp. 496-502.
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