Loss of integrin alpha3 expression associated with acquisition of invasive potential by ovarian clear cell adenocarcinoma cells.

Nao Suzuki, Atsushi Higashiguchi, Yuko Hasegawa, Hiroshi Matsumoto, Shinji Oie, Kimiko Orikawa, Sachiko Ezawa, Nobuyuki Susumu, Ki Ichi Miyashita, Daisuke Aoki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Among various types of surface epithelial ovarian carcinoma, clear cell adenocarcinoma often has a particularly poor prognosis even when diagnosed in stage I. It is resistant to existing anticancer drugs and appears to have different biological properties to other histological types of ovarian cancer. The present study was conducted using cell lines derived from ovarian clear cell adenocarcinoma in order to identify genes associated with the acquisition of malignant potential by this type of cancer. Two cell lines derived from ovarian clear cell adenocarcinoma (RMG-I and RMG-V), with different levels of invasive potential in an invasion assay, were used. DNA fragments were extracted from the band showing differences in the level of expression by RT-PCR with fluorescent differential display. A total of 56 different DNA base sequences were determined by direct sequencing. Primers were established using these base sequences and the level of expression in each cancer cell line was determined by real-time PCR. Integrin a3, the gene of which is present on chromosome 17q, was identified. It was also detected by a microarray analysis as one of the molecules showing a different level of expression between the two cell lines. Then the pattern of integrin a3 expression was investigated using immunocytochemical staining, and was found to differ between the two cell lines. The findings obtained using these cell lines derived from ovarian clear cell adenocarcinoma indicate that integrin alpha3 may associated with the acquisition of malignant potential by clear cell adenocarcinoma.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalHuman cell : official journal of Human Cell Research Society
Volume18
Issue number3
Publication statusPublished - 2005 Sep

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

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    Suzuki, N., Higashiguchi, A., Hasegawa, Y., Matsumoto, H., Oie, S., Orikawa, K., Ezawa, S., Susumu, N., Miyashita, K. I., & Aoki, D. (2005). Loss of integrin alpha3 expression associated with acquisition of invasive potential by ovarian clear cell adenocarcinoma cells. Human cell : official journal of Human Cell Research Society, 18(3), 147-155.