Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells

Tetsutaro Sumiyoshi, Kaoru Sato, Hitomi Yamamoto, Yuka W. Iwasaki, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In Drosophila germ cells, PIWI-interacting RNAs (piR-NAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific pingpong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila.

Original languageEnglish
Pages (from-to)1617-1622
Number of pages6
JournalGenes and Development
Volume30
Issue number14
DOIs
Publication statusPublished - 2016 Jul 15

Keywords

  • CRISPR
  • Drosophila
  • L(3)mbt
  • PIWI
  • PiRNA
  • Ping-pong cycle

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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