Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells

Tetsutaro Sumiyoshi, Kaoru Sato, Hitomi Yamamoto, Yuka Iwasaki, Haruhiko Siomi, Mikiko C. Siomi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In Drosophila germ cells, PIWI-interacting RNAs (piR-NAs) are amplified through a PIWI slicer-dependent feed-forward loop termed the ping-pong cycle, yielding secondary piRNAs. However, the detailed mechanism remains poorly understood, largely because an ex vivo model system amenable to biochemical analyses has not been available. Here, we show that CRISPR-mediated loss of function of lethal (3) malignant brain tumor [l(3)mbt] leads to ectopic activation of the germ-specific pingpong cycle in ovarian somatic cells. Perinuclear foci resembling nuage, the ping-pong center, appeared following l(3)mbt mutation. This activation of the ping-pong machinery in cultured cells will greatly facilitate elucidation of the mechanism underlying secondary piRNA biogenesis in Drosophila.

Original languageEnglish
Pages (from-to)1617-1622
Number of pages6
JournalGenes and Development
Volume30
Issue number14
DOIs
Publication statusPublished - 2016 Jul 15

Fingerprint

Brain Neoplasms
Drosophila
Clustered Regularly Interspaced Short Palindromic Repeats
Menstrual Cycle
Germ Cells
Small Interfering RNA
Cultured Cells
RNA
Mutation

Keywords

  • CRISPR
  • Drosophila
  • L(3)mbt
  • Ping-pong cycle
  • PiRNA
  • PIWI

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells. / Sumiyoshi, Tetsutaro; Sato, Kaoru; Yamamoto, Hitomi; Iwasaki, Yuka; Siomi, Haruhiko; Siomi, Mikiko C.

In: Genes and Development, Vol. 30, No. 14, 15.07.2016, p. 1617-1622.

Research output: Contribution to journalArticle

Sumiyoshi, Tetsutaro ; Sato, Kaoru ; Yamamoto, Hitomi ; Iwasaki, Yuka ; Siomi, Haruhiko ; Siomi, Mikiko C. / Loss of l(3)mbt leads to acquisition of the ping-pong cycle in Drosophila ovarian somatic cells. In: Genes and Development. 2016 ; Vol. 30, No. 14. pp. 1617-1622.
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