Loss of Sprouty4 in T cells ameliorates experimental autoimmune encephalomyelitis in mice by negatively regulating IL-1β receptor expression

Tomohiro Fukaya, Kazue Someya, Sana Hibino, Masahiro Okada, Humitsugu Yamane, Koji Taniguchi, Akihiko Yoshimura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Th17 cells, which have been implicated in autoimmune diseases, require IL-6 and TGF-β for early differentiation. To gain pathogenicity, however, Th17 cells require IL-1β and IL-23. The underlying mechanism by which these confer pathogenicity is not well understood. Here we show that Sprouty4, an inhibitor of the PLCγ-ERK pathway, critically regulates inflammatory Th17 (iTh17) cell differentiation. Sprouty4-deficient mice, as well as mice adoptively transferred with Sprouty4-deficient T cells, were resistant to experimental autoimmune encephalitis (EAE) and showed decreased Th17 cell generation in vivo. In vitro, Sprouty4 deficiency did not severely affect TGF-β/IL-6-induced Th17 cell generation but strongly impaired Th17 differentiation induced by IL-1/IL-6/IL-23. Analysis of Th17-related gene expression revealed that Sprouty4-deficient Th17 cells expressed lower levels of IL-1R1 and IL-23R, while RORγt levels were similar. Consistently, overexpression of Sprouty4 or pharmacological inhibition of ERK upregulated IL-1R1 expression in primary T cells. Thus, Sprouty4 and ERK play a critical role in developing iTh17 cells in Th17 cell-driven autoimmune diseases.

Original languageEnglish
Pages (from-to)471-478
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume447
Issue number3
DOIs
Publication statusPublished - 2014 May 9

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Th17 Cells
Interleukin-1 Receptors
T-cells
Autoimmune Experimental Encephalomyelitis
Interleukin-23
Interleukin-6
T-Lymphocytes
Interleukin-1
Programmable logic controllers
Gene expression
Autoimmune Diseases
Virulence
MAP Kinase Signaling System
Cell Differentiation
Pharmacology
Gene Expression

Keywords

  • Cytokine signaling
  • ERK
  • Helper T cells
  • IL-1 receptor
  • Th17

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Loss of Sprouty4 in T cells ameliorates experimental autoimmune encephalomyelitis in mice by negatively regulating IL-1β receptor expression. / Fukaya, Tomohiro; Someya, Kazue; Hibino, Sana; Okada, Masahiro; Yamane, Humitsugu; Taniguchi, Koji; Yoshimura, Akihiko.

In: Biochemical and Biophysical Research Communications, Vol. 447, No. 3, 09.05.2014, p. 471-478.

Research output: Contribution to journalArticle

Fukaya, Tomohiro ; Someya, Kazue ; Hibino, Sana ; Okada, Masahiro ; Yamane, Humitsugu ; Taniguchi, Koji ; Yoshimura, Akihiko. / Loss of Sprouty4 in T cells ameliorates experimental autoimmune encephalomyelitis in mice by negatively regulating IL-1β receptor expression. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 447, No. 3. pp. 471-478.
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