Low-dose ethanol attenuates gut ischemia/reperfusion-induced liver injury in rats via nitric oxide production

Yoshinori Horie, Yoshiyuki Yamagishi, Shinzo Kato, Mikio Kajihara, Hiroyuki Kimura, Hiromasa Ishii

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background and Aims: The acute administration of low-dose ethanol was demonstrated to attenuate liver injury elicited by gut ischemia/reperfusion (I/R). Nitric oxide (NO) has been found to be a modulator of adhesive interactions between leukocytes, platelets, and endothelial cells, but there has been much controversy about the effects of ethanol on NO regulation. The objective of this study was to investigate the role of NO in ethanol-reduced hepatic microvascular dysfunction elicited by gut I/R. Methods: Male Wistar rats were exposed to 30 min of gut ischemia followed by 60 min of reperfusion. Intravital microscopy was used to monitor leukocyte recruitment and non-perfused sinusoids (NPS). Plasma alanine aminotransferase (ALT) activities were measured 6 h after the onset of reperfusion. In another set of experiments, ethanol (10%, 1 g/kg) was administered before ischemia. Results: Gut I/R elicited increases in the number of stationary leukocytes, NPS, and plasma ALT activities; all of which were attenuated by pretreatment with ethanol or an NO donor. Gut I/R caused the apoptosis of hepatocytes, which was prevented by pretreatment with ethanol. Pretreatment with an NO synthase inhibitor diminished the protective effects of ethanol. The administration of ethanol increased plasma nitrite/nitrate levels. Conclusion: These results suggest that low-dose ethanol attenuates the gut I/R-induced hepatic microvascular dysfunction and sequential liver injury by increasing sinusoidal NO levels.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume18
Issue number2
DOIs
Publication statusPublished - 2003 Feb

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Keywords

  • Intravital microscope
  • Nitric oxide synthase
  • Tissue hypoxia

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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