LPP expression during in vitro smooth muscle differentiation and stent-induced vascular injury

I. Gorenne, L. Jin, Tadashi Yoshida, J. M. Sanders, I. J. Sarembock, G. K. Owens, A. P. Somlyo, A. V. Somlyo

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Lipoma preferred partner (LPP) has been identified as a protein highly expressed in smooth muscle (SM) tissues. The aim of the present study was to determine mechanisms that regulate LPP expression in an in vitro model of SM cell (SMC) differentiation and in stent-induced pig coronary vessel injury. All trans-retinoic acid treatment of A404 cells induced a strong increase in LPP, as well as SM α-actin, SM myosin heavy chain, and smoothelin mRNA levels, in a Rho kinase (ROK)-dependent manner. Adenovirus mediated overexpression of myocardin in A404 cells significantly increased LPP mRNA expression. Interestingly, inactivation of RhoA with C3-exoenzyme or treatment with ROK inhibitors strongly inhibited myocardin mRNA expression in retinoic acid-treated A404 cells or human iliac vein SMCs. LPP silencing with short interfering RNA significantly decreased SMC migration. LPP expression was also markedly decreased in focal adhesion kinase (FAK)-null cells known to have impaired migration but rescued with inducible expression of FAK. LPP expression in FAK-null fibroblasts enhanced cell spreading. In stented pig coronary vessels, LPP was expressed in the neointima of cells lacking smoothelin and showed expression patterns identical to those of SM α-actin. In conclusion, LPP appears to be a myocardin-, RhoA/ROK-dependent SMC differentiation marker that plays a role in regulating SMC migration.

Original languageEnglish
Pages (from-to)378-385
Number of pages8
JournalCirculation Research
Volume98
Issue number3
DOIs
Publication statusPublished - 2006 Feb
Externally publishedYes

Fingerprint

Lipoma
Vascular System Injuries
Stents
Smooth Muscle
Focal Adhesion Protein-Tyrosine Kinases
rho-Associated Kinases
Tretinoin
Messenger RNA
Cell Movement
Actins
Cell Differentiation
Coronary Vessels
Swine
Smooth Muscle Myosins
Iliac Vein
In Vitro Techniques
Neointima
Null Lymphocytes
Myosin Heavy Chains
Differentiation Antigens

Keywords

  • Cell migration
  • Focal adhesion kinase
  • Lipoma preferred partner
  • Smooth muscle
  • Vascular injury

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

LPP expression during in vitro smooth muscle differentiation and stent-induced vascular injury. / Gorenne, I.; Jin, L.; Yoshida, Tadashi; Sanders, J. M.; Sarembock, I. J.; Owens, G. K.; Somlyo, A. P.; Somlyo, A. V.

In: Circulation Research, Vol. 98, No. 3, 02.2006, p. 378-385.

Research output: Contribution to journalArticle

Gorenne, I, Jin, L, Yoshida, T, Sanders, JM, Sarembock, IJ, Owens, GK, Somlyo, AP & Somlyo, AV 2006, 'LPP expression during in vitro smooth muscle differentiation and stent-induced vascular injury', Circulation Research, vol. 98, no. 3, pp. 378-385. https://doi.org/10.1161/01.RES.0000202802.34727.fd
Gorenne, I. ; Jin, L. ; Yoshida, Tadashi ; Sanders, J. M. ; Sarembock, I. J. ; Owens, G. K. ; Somlyo, A. P. ; Somlyo, A. V. / LPP expression during in vitro smooth muscle differentiation and stent-induced vascular injury. In: Circulation Research. 2006 ; Vol. 98, No. 3. pp. 378-385.
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