Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

Ryo Nishihara, Masahiro Abe, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.

Original languageEnglish
Article number955
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 2017 Dec 1

Fingerprint

Luciferases
Biological Assay
Optical Imaging
Sirolimus
Fluorescence
coelenterazine

ASJC Scopus subject areas

  • General

Cite this

Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays. / Nishihara, Ryo; Abe, Masahiro; Nishiyama, Shigeru; Citterio, Daniel; Suzuki, Koji; Kim, Sung Bae.

In: Scientific Reports, Vol. 7, No. 1, 955, 01.12.2017.

Research output: Contribution to journalArticle

Nishihara, Ryo ; Abe, Masahiro ; Nishiyama, Shigeru ; Citterio, Daniel ; Suzuki, Koji ; Kim, Sung Bae. / Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
@article{a736ed2180134a77824a5c24858dc4b3,
title = "Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays",
abstract = "Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.",
author = "Ryo Nishihara and Masahiro Abe and Shigeru Nishiyama and Daniel Citterio and Koji Suzuki and Kim, {Sung Bae}",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-00955-6",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

AU - Nishihara, Ryo

AU - Abe, Masahiro

AU - Nishiyama, Shigeru

AU - Citterio, Daniel

AU - Suzuki, Koji

AU - Kim, Sung Bae

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.

AB - Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luciferase selectivity. In the attempt, we found that CTZ analogues with ethynyl or styryl groups display dramatically biased bioluminescence to specific luciferases and pHs by modifying the functional groups at the C-2 and C-6 positions of the imidazopyradinone backbone of CTZ. The optical contamination-free feature was exemplified with the luciferase-specific CTZ analogues, which illuminated anti-estrogenic and rapamycin activities in a mixture of optical probes. This unique bioluminescence platform has great potential for specific and high throughput imaging of multiple optical readouts in bioassays without optical contamination.

UR - http://www.scopus.com/inward/record.url?scp=85018399801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018399801&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-00955-6

DO - 10.1038/s41598-017-00955-6

M3 - Article

C2 - 28424463

AN - SCOPUS:85018399801

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 955

ER -