Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore - role of degranulation and myeloperoxidase

Makoto Suematsu, Chikara Oshio, Soichiro Miura, Masayuki Suzuki, Shigenari Houzawa, Masaharu Tsuchiya

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 μg/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 μg/ml). The additional application of 0.1 μM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the distribution of neutrophils, and these photonic activities were gradually spread to extracellular space. Sodium azide, which prevents myeloperoxidase activity, inhibited Ca2+ ionophore-induced photonic burst from phorbol ester-treated neutrophil. These findings suggest a prerequisite role of degranulation and myeloperoxidase release in luminol-dependent photoemission from stimulated neutrophils.

Original languageEnglish
Pages (from-to)106-111
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number1
DOIs
Publication statusPublished - 1988 Aug 30

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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