TY - JOUR
T1 - Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore - role of degranulation and myeloperoxidase
AU - Suematsu, Makoto
AU - Oshio, Chikara
AU - Miura, Soichiro
AU - Suzuki, Masayuki
AU - Houzawa, Shigenari
AU - Tsuchiya, Masaharu
N1 - Funding Information:
The authors thank Prof. Y. Ishimura and Prof. S. Kanegasaki for their kind suggestions. This work is supported by the grant from Japanese Ministry of Education No. 63440033 and by the grant from Keio University, School of Medicine.
PY - 1988/8/30
Y1 - 1988/8/30
N2 - Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 μg/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 μg/ml). The additional application of 0.1 μM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the distribution of neutrophils, and these photonic activities were gradually spread to extracellular space. Sodium azide, which prevents myeloperoxidase activity, inhibited Ca2+ ionophore-induced photonic burst from phorbol ester-treated neutrophil. These findings suggest a prerequisite role of degranulation and myeloperoxidase release in luminol-dependent photoemission from stimulated neutrophils.
AB - Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 μg/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 μg/ml). The additional application of 0.1 μM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the distribution of neutrophils, and these photonic activities were gradually spread to extracellular space. Sodium azide, which prevents myeloperoxidase activity, inhibited Ca2+ ionophore-induced photonic burst from phorbol ester-treated neutrophil. These findings suggest a prerequisite role of degranulation and myeloperoxidase release in luminol-dependent photoemission from stimulated neutrophils.
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U2 - 10.1016/S0006-291X(88)81055-6
DO - 10.1016/S0006-291X(88)81055-6
M3 - Article
C2 - 2843175
AN - SCOPUS:0023773306
SN - 0006-291X
VL - 155
SP - 106
EP - 111
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -