Lung fibroblasts accelerate wound closure in human alveolar epithelial cells through hepatocyte growth factor/c-Met signaling

Yoko Ito, Kelly Correll, John A. Schiel, Jay H. Finigan, Rytis Prekeris, Robert J. Mason

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

There are 190,600 cases of acute lung injury/acute respiratory distress syndrome (ALI/ARDS) each year in the United States, and the incidence and mortality of ALI/ARDS increase dramatically with age. Patients with ALI/ARDS have alveolar epithelial injury, which may be worsened by high-pressure mechanical ventilation. Alveolar type II (ATII) cells are the progenitor cells for the alveolar epithelium and are required to reestablish the alveolar epithelium during the recovery process from ALI/ARDS. Lung fibroblasts (FBs) migrate and proliferate early after lung injury and likely are an important source of growth factors for epithelial repair. However, how lung FBs affect epithelial wound healing in the human adult lung has not been investigated in detail. Hepatocyte growth factor (HGF) is known to be released mainly from FBs and to stimulate both migration and proliferation of primary rat ATII cells. HGF is also increased in lung tissue, bronchoalveolar lavage fluid, and serum in patients with ALI/ARDS. Therefore, we hypothesized that HGF secreted by FBs would enhance wound closure in alveolar epithelial cells (AECs). Wound closure was measured using a scratch wound-healing assay in primary human AEC monolayers and in a coculture system with FBs. We found that wound closure was accelerated by FBs mainly through HGF/c-Met signaling. HGF also restored impaired wound healing in AECs from the elderly subjects and after exposure to cyclic stretch. We conclude that HGF is the critical factor released from FBs to close wounds in human AEC monolayers and suggest that HGF is a potential strategy for hastening alveolar repair in patients with ALI/ARDS.

Original languageEnglish
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume307
Issue number1
DOIs
Publication statusPublished - 2014 Jul 1

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Alveolar Epithelial Cells
Hepatocyte Growth Factor
Acute Lung Injury
Adult Respiratory Distress Syndrome
Fibroblasts
Lung
Wounds and Injuries
Wound Healing
Epithelium
Bronchoalveolar Lavage Fluid
Lung Injury
Coculture Techniques
Artificial Respiration
Intercellular Signaling Peptides and Proteins
Stem Cells
Pressure
Mortality
Incidence
Serum

Keywords

  • Alveolar epithelial cells
  • Hepatocyte growth factor
  • Lung fibroblasts
  • Wound closure

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology
  • Physiology

Cite this

Lung fibroblasts accelerate wound closure in human alveolar epithelial cells through hepatocyte growth factor/c-Met signaling. / Ito, Yoko; Correll, Kelly; Schiel, John A.; Finigan, Jay H.; Prekeris, Rytis; Mason, Robert J.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 307, No. 1, 01.07.2014.

Research output: Contribution to journalArticle

Ito, Yoko ; Correll, Kelly ; Schiel, John A. ; Finigan, Jay H. ; Prekeris, Rytis ; Mason, Robert J. / Lung fibroblasts accelerate wound closure in human alveolar epithelial cells through hepatocyte growth factor/c-Met signaling. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2014 ; Vol. 307, No. 1.
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