Lutein acts via multiple antioxidant pathways in the photo-stressed retina

Mamoru Kamoshita, Eriko Toda, Hideto Osada, Toshio Narimatsu, Saori Kobayashi, Kazuo Tsubota, Yoko Ozawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Lutein slows the progression of age-related macular degeneration (AMD), a leading cause of blindness in ageing societies. However, the underlying mechanisms remain elusive. Here, we evaluated lutein's effects on light-induced AMD-related pathological events. Balb/c mice exposed to light (2000 lux, 3 h) showed tight junction disruption in the retinal pigment epithelium (RPE) at 12 h, as detected by zona occludens-1 immunostaining. Substantial disruption remained 48 h after light exposure in the vehicle-treated group; however, this was ameliorated in the mice treated with intraperitoneal lutein at 12 h, suggesting that lutein promoted tight junction repair. In the photo-stressed RPE and the neighbouring choroid tissue, lutein suppressed reactive oxygen species and increased superoxide dismutase (SOD) activity at 24 h, and produced sustained increases in sod1 and sod2 mRNA levels at 48 h. SOD activity was induced by lutein in an RPE cell line, ARPE19. We also found that lutein suppressed upregulation of macrophage-related markers, f4/80 and mcp-1, in the RPE-choroid tissue at 18 h. In ARPE19, lutein reduced mcp-1 mRNA levels. These findings indicated that lutein promoted tight junction repair and suppressed inflammation in photo-stressed mice, reducing local oxidative stress by direct scavenging and most likely by induction of endogenous antioxidant enzymes.

Original languageEnglish
Article number30226
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2016 Jul 22

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Lutein
Retina
Antioxidants
Retinal Pigment Epithelium
Tight Junctions
Choroid
Macular Degeneration
Light
Superoxide Dismutase
Messenger RNA
Herpes Zoster
Blindness
Reactive Oxygen Species
Oxidative Stress
Up-Regulation
Macrophages
Inflammation
Cell Line

ASJC Scopus subject areas

  • General

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Lutein acts via multiple antioxidant pathways in the photo-stressed retina. / Kamoshita, Mamoru; Toda, Eriko; Osada, Hideto; Narimatsu, Toshio; Kobayashi, Saori; Tsubota, Kazuo; Ozawa, Yoko.

In: Scientific Reports, Vol. 6, 30226, 22.07.2016.

Research output: Contribution to journalArticle

Kamoshita, Mamoru ; Toda, Eriko ; Osada, Hideto ; Narimatsu, Toshio ; Kobayashi, Saori ; Tsubota, Kazuo ; Ozawa, Yoko. / Lutein acts via multiple antioxidant pathways in the photo-stressed retina. In: Scientific Reports. 2016 ; Vol. 6.
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