M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

H. Wu, M. Durante, Y. Furusawa, K. George, T. Kawata, F. A. Cucinotta

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

Original languageEnglish
Pages (from-to)1537-1542
Number of pages6
JournalAdvances in Space Research
Volume31
Issue number6
DOIs
Publication statusPublished - 2003 Mar
Externally publishedYes

Fingerprint

chromosome aberrations
fibroblasts
fishes
Fibroblasts
Chromosomes
Aberrations
Fish
chromosomes
chromosome
energetics
Iron
iron
ion
Ions
fish
ions
aberration
dosage
Committee on Space Research
repair

ASJC Scopus subject areas

  • Space and Planetary Science
  • Astronomy and Astrophysics

Cite this

M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro. / Wu, H.; Durante, M.; Furusawa, Y.; George, K.; Kawata, T.; Cucinotta, F. A.

In: Advances in Space Research, Vol. 31, No. 6, 03.2003, p. 1537-1542.

Research output: Contribution to journalArticle

Wu, H. ; Durante, M. ; Furusawa, Y. ; George, K. ; Kawata, T. ; Cucinotta, F. A. / M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro. In: Advances in Space Research. 2003 ; Vol. 31, No. 6. pp. 1537-1542.
@article{3374264449044a4a9241fe7dbed0f707,
title = "M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro",
abstract = "Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.",
author = "H. Wu and M. Durante and Y. Furusawa and K. George and T. Kawata and Cucinotta, {F. A.}",
year = "2003",
month = "3",
doi = "10.1016/S0273-1177(03)00092-9",
language = "English",
volume = "31",
pages = "1537--1542",
journal = "Advances in Space Research",
issn = "0273-1177",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

AU - Wu, H.

AU - Durante, M.

AU - Furusawa, Y.

AU - George, K.

AU - Kawata, T.

AU - Cucinotta, F. A.

PY - 2003/3

Y1 - 2003/3

N2 - Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

AB - Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

UR - http://www.scopus.com/inward/record.url?scp=0037623774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037623774&partnerID=8YFLogxK

U2 - 10.1016/S0273-1177(03)00092-9

DO - 10.1016/S0273-1177(03)00092-9

M3 - Article

C2 - 12971408

AN - SCOPUS:0037623774

VL - 31

SP - 1537

EP - 1542

JO - Advances in Space Research

JF - Advances in Space Research

SN - 0273-1177

IS - 6

ER -