M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

H. Wu; M. Durante; Y. Furusawa; K. George; T. Kawata; F. A. Cucinotta

doi:10.1016/S0273-1177(03)00092-9

M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

H. Wu, M. Durante, Y. Furusawa, K. George, T. Kawata, F. A. Cucinotta

Department of Radiology

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

Original language	English
Pages (from-to)	1537-1542
Number of pages	6
Journal	Advances in Space Research
Volume	31
Issue number	6
DOIs	https://doi.org/10.1016/S0273-1177(03)00092-9
Publication status	Published - 2003 Mar

ASJC Scopus subject areas

Aerospace Engineering
Astronomy and Astrophysics
Geophysics
Atmospheric Science
Space and Planetary Science
Earth and Planetary Sciences(all)

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10.1016/S0273-1177(03)00092-9

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title = "M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro",

abstract = "Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.",

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T1 - M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

AU - Wu, H.

AU - Durante, M.

AU - Furusawa, Y.

AU - George, K.

AU - Kawata, T.

AU - Cucinotta, F. A.

PY - 2003/3

Y1 - 2003/3

N2 - Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

AB - Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

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M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

Abstract

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