M-fish analysis of chromosome aberrations in human fibroblasts exposed to energetic iron ions in vitro

H. Wu, M. Durante, Y. Furusawa, K. George, T. Kawata, F. A. Cucinotta

Research output: Contribution to journalArticle

14 Citations (Scopus)


Confluent human fibroblast cells were exposed to 6 Gy γ-rays or 200 MeV/nucleon Fe ions at 0.7 or 3 Gy. The cells were allowed to repair for 24 hours after exposure and chromosomes were collected using a premature chromosome condensation technique with calyculin-A. Chromosome aberrations were analyzed using the multicolor FISH (mFISH) technique that allows identification of both complex and truly incomplete exchanges. Results showed that both doses of the Fe ions produced higher ratios of complex to simple exchanges and lower ratio of complete to incomplete exchanges than the 6 Gy γ-exposure. The ratios of aberration yields were similar for the two doses of Fe ions. After 0.7 Gy of Fe ions, most complex aberrations were found to involve three or four chromosomes, indicating this is the maximum number of chromosome domains traversed by a single Fe ion track. Published by Elsevier Sciencce Ltd on behalf of COSPAR.

Original languageEnglish
Pages (from-to)1537-1542
Number of pages6
JournalAdvances in Space Research
Issue number6
Publication statusPublished - 2003 Mar


ASJC Scopus subject areas

  • Aerospace Engineering
  • Astronomy and Astrophysics
  • Geophysics
  • Atmospheric Science
  • Space and Planetary Science
  • Earth and Planetary Sciences(all)

Cite this