Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant

Gaku Kumano, Narudo Kawai, Hiroki Nishida

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The anterior-posterior (A-P) axis in ascidian embryos is established through the posteriorizing activities of a localized egg region known as the posterior vegetal cortex/cytoplasm (PVC). Here we describe a novel function of macho-1, a maternally-localized muscle determinant, in establishment of the A-P axis in the Halocynthia roretzi embryo. Macho-1, in addition to its known function in the formation of posterior tissue such as muscle and mesenchyme, and suppression of the anterior-derived notochord fate, acts independently of its transcriptional activity as a regulator of posterior-specific unequal cell divisions, in cooperation with β-catenin. Our results suggest that macho-1 and β-catenin regulate the formation of a microtubule bundle that shortens and pulls the centrosome toward a sub-cellular cortical structure known as centrosome-attracting body (CAB), which is located at the posterior pole of the embryo during unequal cell divisions, and act upstream of PEM, a recently-identified regulator of unequal cell divisions. We also present data that suggest that PEM localization to the CAB may not be required for unequal cleavage regulation. The present study provides an important and novel insight into the role of the zinc-finger-containing transcription factor and indicates that it constitutes a major part of the PVC activity.

Original languageEnglish
Pages (from-to)284-292
Number of pages9
JournalDevelopmental Biology
Volume344
Issue number1
DOIs
Publication statusPublished - 2010 Aug 1
Externally publishedYes

Fingerprint

Centrosome
Cell Division
Catenins
Embryonic Structures
Muscles
Cytoplasm
Notochord
Urochordata
Zinc Fingers
Mesoderm
Cellular Structures
Microtubules
Ovum
Transcription Factors

Keywords

  • β-catenin
  • Anterior-posterior axis
  • Ascidian
  • Macho-1
  • PEM
  • Unequal cell division

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant. / Kumano, Gaku; Kawai, Narudo; Nishida, Hiroki.

In: Developmental Biology, Vol. 344, No. 1, 01.08.2010, p. 284-292.

Research output: Contribution to journalArticle

Kumano, Gaku ; Kawai, Narudo ; Nishida, Hiroki. / Macho-1 regulates unequal cell divisions independently of its function as a muscle determinant. In: Developmental Biology. 2010 ; Vol. 344, No. 1. pp. 284-292.
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