La presenza in coltura di macrophage inflammatory protein-1α (MIP-1α) o di leukemia inhibitory factor (LIF) protegge la capacità delle cellule staminali marine purificate di colonizzare il midollo dopo trapianto

Translated title of the contribution: Macrophage inflammatory protein-1α (MIP-1α) and leukemia inhibitory factor (LIF) protect the repopulating ability of purified murine hematopoietic stem cells in serum-deprived cultures stimulated with SCF and IL-3

Ryuji Tanosaki, Eishi Ashihara, Laura Alessandrini, Giovanni Migliaccio, Anna Rita Migliaccio

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1α or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1α and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.

Original languageItalian
Pages (from-to)553-562
Number of pages10
JournalAnnali dell'Istituto Superiore di Sanita
Volume35
Issue number4
Publication statusPublished - 1999 Dec 1
Externally publishedYes

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Keywords

  • Bone marrow transplantation
  • Ex vivo expansion
  • Hematopoietic stem cells
  • Leukemia inhibitory factor
  • Macrophage inflammatory protein-1α

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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