La presenza in coltura di macrophage inflammatory protein-1α (MIP-1α) o di leukemia inhibitory factor (LIF) protegge la capacità delle cellule staminali marine purificate di colonizzare il midollo dopo trapianto

Translated title of the contribution: Macrophage inflammatory protein-1α (MIP-1α) and leukemia inhibitory factor (LIF) protect the repopulating ability of purified murine hematopoietic stem cells in serum-deprived cultures stimulated with SCF and IL-3

Ryuji Tanosaki, Eishi Ashihara, Laura Alessandrini, Giovanni Migliaccio, Anna Rita Migliaccio

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1α or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1α and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.

Original languageItalian
Pages (from-to)553-562
Number of pages10
JournalAnnali dell'Istituto Superiore di Sanita
Volume35
Issue number4
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Macrophage Inflammatory Proteins
Leukemia Inhibitory Factor
Interleukin-3
Hematopoietic Stem Cells
Stem Cells
Serum
Stem Cell Factor
Electrophoresis
Hemoglobins
Leukocytes
Transplantation
Erythrocytes
Polymerase Chain Reaction

Keywords

  • Bone marrow transplantation
  • Ex vivo expansion
  • Hematopoietic stem cells
  • Leukemia inhibitory factor
  • Macrophage inflammatory protein-1α

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

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title = "La presenza in coltura di macrophage inflammatory protein-1α (MIP-1α) o di leukemia inhibitory factor (LIF) protegge la capacit{\`a} delle cellule staminali marine purificate di colonizzare il midollo dopo trapianto",
abstract = "The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1α or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1α and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.",
keywords = "Bone marrow transplantation, Ex vivo expansion, Hematopoietic stem cells, Leukemia inhibitory factor, Macrophage inflammatory protein-1α",
author = "Ryuji Tanosaki and Eishi Ashihara and Laura Alessandrini and Giovanni Migliaccio and Migliaccio, {Anna Rita}",
year = "1999",
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pages = "553--562",
journal = "Annali dell'Istituto Superiore di Sanita",
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TY - JOUR

T1 - La presenza in coltura di macrophage inflammatory protein-1α (MIP-1α) o di leukemia inhibitory factor (LIF) protegge la capacità delle cellule staminali marine purificate di colonizzare il midollo dopo trapianto

AU - Tanosaki, Ryuji

AU - Ashihara, Eishi

AU - Alessandrini, Laura

AU - Migliaccio, Giovanni

AU - Migliaccio, Anna Rita

PY - 1999

Y1 - 1999

N2 - The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1α or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1α and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.

AB - The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1α or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1α and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.

KW - Bone marrow transplantation

KW - Ex vivo expansion

KW - Hematopoietic stem cells

KW - Leukemia inhibitory factor

KW - Macrophage inflammatory protein-1α

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